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Related Experiment Video

Updated: May 21, 2026

Fabrication of Extracellular Matrix-derived Foams and Microcarriers as Tissue-specific Cell Culture and Delivery Platforms
11:19

Fabrication of Extracellular Matrix-derived Foams and Microcarriers as Tissue-specific Cell Culture and Delivery Platforms

Published on: April 11, 2017

Cryopreservation of cell/scaffold tissue-engineered constructs.

Pedro F Costa1, Ana F Dias, Rui L Reis

  • 13B's Research Group - Biomaterials, Biodegradables and Biomimetics, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, University of Minho, Guimarães, Portugal.

Tissue Engineering. Part C, Methods
|June 9, 2012
PubMed
Summary
This summary is machine-generated.

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Cryopreservation of tissue-engineered constructs using starch scaffolds and goat bone marrow stem cells is feasible. Porous scaffolds enhance cell viability during cryopreservation, enabling off-the-shelf tissue substitutes.

Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Cell Biology

Background:

  • Tissue-engineered constructs require preservation methods for clinical application.
  • Cryopreservation is a potential strategy for long-term storage of engineered tissues.
  • Evaluating cryopreservation's impact on both cells and scaffold properties is crucial.

Purpose of the Study:

  • To investigate the effect of cryopreservation on the functionality of tissue-engineered constructs.
  • To assess cell viability and scaffold properties after cryopreservation.
  • To determine the influence of scaffold architecture on cryopreservation outcomes.

Main Methods:

  • Tissue-engineered constructs were fabricated using starch/poly(caprolactone) fiber-mesh scaffolds.
  • Goat bone marrow stem cells (GBMSCs) were seeded onto scaffolds and cryopreserved for 7 days.

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Tissue Engineering: Construction of a Multicellular 3D Scaffold for the Delivery of Layered Cell Sheets
09:24

Tissue Engineering: Construction of a Multicellular 3D Scaffold for the Delivery of Layered Cell Sheets

Published on: October 3, 2014

Related Experiment Videos

Last Updated: May 21, 2026

Fabrication of Extracellular Matrix-derived Foams and Microcarriers as Tissue-specific Cell Culture and Delivery Platforms
11:19

Fabrication of Extracellular Matrix-derived Foams and Microcarriers as Tissue-specific Cell Culture and Delivery Platforms

Published on: April 11, 2017

Tissue Engineering: Construction of a Multicellular 3D Scaffold for the Delivery of Layered Cell Sheets
09:24

Tissue Engineering: Construction of a Multicellular 3D Scaffold for the Delivery of Layered Cell Sheets

Published on: October 3, 2014

  • Scaffold properties and cell viability were analyzed pre- and post-cryopreservation, with nonporous discs as controls.

    • Main Results:

    • Standard cryopreservation methods successfully maintained cell viability and scaffold properties.
    • Porous scaffolds demonstrated superior retention of cellular content and viability compared to nonporous discs.
    • Scaffold porosity and interconnectivity positively influenced cell survival during cryopreservation.

    Conclusions:

    • Cryopreservation is a viable method for preserving tissue-engineered constructs made with starch scaffolds and GBMSCs.
    • Scaffold design, particularly porosity and interconnectivity, is critical for successful cryopreservation.
    • These findings support the development of off-the-shelf engineered tissue substitutes for immediate clinical availability.