Related Experiment Video
Updated: May 21, 2026

Constructing Mutants in Serotype 1 Streptococcus pneumoniae strain 519/43
Published on: September 11, 2020
[Construction and activities of suilysin mutants]
Zhi-qiang Ren1, Yu-ling Zheng, Shu-zhen Gan
1College of Life Science, Sichuan Normal University, Chengdu, China. renzhiq@qq.com
Aim:
To construct the suilysin mutant without hemolytic activity and evaluate its functions.
Methods:
The proline in 353 site of suilysin was site-directed mutated to alanine, leucine and valine, respectively. The recombinant mutants were renaturated and purified by immobilized metal ion affinity chromatography, and the purified proteins were evaluated in the hemolytic activity and immunogenicity.
Results:
We obtained three mutants, SLY(P353A), SLY(P353L) and SLY(P353V). The SLY(P353V) mutant had non-hemolytic activity. Western blotting and animal experiments showed that SLY(P353V) mutant still had immunogenicity.
Conclusion:
Suilysin mutant SLY(P353V) has no hemolytic activity but remains immunogenicity.
Related Concept Videos
Pleiotropy
Conservative Site-specific Recombination and Phase Variation
The recognition sites for Cre recombinase called LoxP...

