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[Characteristics of Bordetelia pertussis strains isolated from pertussis patients in Moscow by using multilocus

O Iu borisova, I K Mazurova, G A Ivashinnikova

    Zhurnal Mikrobiologii, Epidemiologii I Immunobiologii
    |June 15, 2012
    PubMed
    Abstract

    Insights

    Genotyping of Bordetella pertussis (B. pertussis) strains revealed significant genetic differences between contemporary strains causing pertussis in Moscow and those used in the DTP vaccine. This highlights evolving bacterial populations.

    Area of Science:

    • Microbiology
    • Genetics
    • Epidemiology

    Background:

    • Pertussis remains a significant public health concern.
    • Understanding the genetic diversity of Bordetella pertussis is crucial for vaccine efficacy.
    • Previous studies have not fully characterized contemporary B. pertussis strains in Moscow.

    Purpose of the Study:

    • To genotype B. pertussis strains isolated from pertussis patients in Moscow.
    • To compare the genetic profiles of circulating strains with vaccine strains.
    • To assess the clonal structure of contemporary B. pertussis.

    Main Methods:

    • Multilocus sequence typing (MLST) was employed.
    • Allele combinations of ptxA, ptxC, and tcfA genes were analyzed.
    • 53 clinical isolates and 3 vaccine strains were studied.

    Main Results:

    • Four sequence types (ST1, ST2, ST3, ST5) were identified among clinical isolates.
    • The majority of circulating strains (86.7%) belonged to ST5.
    • Vaccine strains were predominantly ST1 and ST2.

    Conclusions:

    • Contemporary B. pertussis strains in Moscow exhibit a different clonal structure compared to vaccine strains.
    • The genetic divergence suggests potential implications for DTP vaccine effectiveness.
    • Further surveillance of B. pertussis genotypes is warranted.

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