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Hepatic nitrosative stress in experimental diabetes
Fábio Cangeri Di Naso1, Graziella Rodrigues, Alexandre Simões Dias
1Laboratório de Fisiologia e Gastroenterologia Experimental, Hospital de Clínicas de Porto Alegre, Universidade Federal do Rio Grande do Sul (UFRGS) 90035-903 Porto Alegre, RS, Brazil.
Journal of Diabetes and Its Complications
|June 16, 2012
Summary
Aminoguanidine effectively reduced nitrosative stress and liver damage in diabetic rats. This treatment lowered markers of oxidative stress, including lipoperoxidation and specific protein expressions, in the diabetic liver.
Area of Science:
- Biomedical Science
- Pharmacology
- Diabetology
Background:
- Diabetes mellitus (DM) is associated with increased oxidative and nitrosative stress.
- Nitrosative stress contributes to cellular damage in diabetic conditions.
Purpose of the Study:
- To investigate the effects of aminoguanidine on nitrosative stress in an experimental model of diabetes mellitus.
- To evaluate aminoguanidine's impact on oxidative stress markers and specific protein expressions in the liver of diabetic rats.
Main Methods:
- Wistar rats were divided into control, diabetic, and aminoguanidine-treated diabetic groups.
- Aminoguanidine (50 mg/kg) was administered intraperitoneally for the final 30 days.
- Liver expression levels of lipoperoxidation (TBARS), inducible nitric oxide synthase (iNOS), nitrotyrosine, and NFκB p65 were analyzed via western blot.
Main Results:
- Diabetic rats exhibited elevated lipoperoxidation and increased expression of iNOS, nitrotyrosine, and NFκB p65.
- Aminoguanidine treatment significantly reduced hepatic lipid peroxidation.
- Aminoguanidine decreased the protein expression of iNOS, nitrotyrosine, and NFκB p65 in diabetic livers.
Conclusions:
- Aminoguanidine treatment ameliorates liver oxidative and nitrosative stress in a rat model of diabetes mellitus.
- The compound effectively reduced the expression of key stress-related proteins, including NFκB p65, in the diabetic liver.
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