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Alternative RNA Splicing

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...

Alternative RNA Splicing

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...

RNA Splicing

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...

RNA Splicing

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...

What is Gene Expression?

What is Gene Expression?

A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is comprised of nucleotides and proteins are comprised of amino acids, a mediator is required to convert the information encoded in DNA into proteins. This mediator is the messenger RNA (mRNA). mRNA copies the blueprint from DNA by a process called transcription. In eukaryotes, transcription occurs in the nucleus by complementary base-pairing with the DNA template. The mRNA is then processed and...

Chromatin Structure Regulates pre-mRNA Processing

Chromatin Structure Regulates pre-mRNA Processing

In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...

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Related Experiment Video

Updated: May 21, 2026

Detection of Alternative Splicing During Epithelial-Mesenchymal Transition

Detection of Alternative Splicing During Epithelial-Mesenchymal Transition

Published on: October 9, 2014

Low dose proteasome inhibition affects alternative splicing.

Sven Bieler¹, Elke Hammer, Manuela Gesell-Salazar

¹Medizinische Klinik mit Schwerpunkt Kardiologie und Angiologie, Charité-Universitätsmedizin, Berlin, Germany.

Journal of Proteome Research

|June 19, 2012

Summary

Low dose proteasome inhibition triggers a protective stress response by altering nuclear regulators. This affects posttranscriptional regulation, impacting alternative splicing of key stress-associated genes.

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Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells

Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells

Published on: April 26, 2017

Area of Science:

Cellular Biology
Molecular Biology
Proteomics

Background:

The ubiquitin proteasome system regulates protein degradation, influencing cellular functions.
Proteasome inhibition causes dose-dependent cellular effects, including apoptosis and stress responses.

Purpose of the Study:

Identify nuclear regulators involved in the protective stress response to low-dose proteasome inhibition.
Investigate the impact of proteasome inhibition on posttranscriptional regulation and alternative splicing.

Main Methods:

Primary human endothelial cells treated with MG132 (proteasome inhibitor).
Proteomic analysis of nuclear extracts using 2-D differential in gel electrophoresis (DIGE).
Affymetrix exon array profiling to assess alternative splicing patterns.

Main Results:

Over 24 splice factors were differentially regulated by low-dose proteasome inhibition.
Increased levels of hnRNPA1 isoforms suggest altered posttranslational modification.
Alternative RNA processing observed for stress-associated genes like caspases and heat shock proteins.

Conclusions:

Low-dose proteasome inhibition impacts posttranscriptional regulation of splice factors.
Early alternative splicing events are affected by proteasome inhibition.
This study provides novel insights into cellular stress responses at the posttranscriptional level.