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Temperature-dependent antibody kinetics as a tool in antibody lead selection
Michael Schräml1, Leopold von Proff
1Roche Diagnostics GmbH, Penzberg, Germany. michael.schraeml@roche.com
Abstract:
Antibody-antigen interactions can principally be classified into three different temperature-dependent kinetic rate profiles. The affinity K (D) can persist, decrease, or increase in the temperature gradient. Today, the impact of temperature-dependent antibody kinetics is recognized, especially as part of the development of best in class monoclonal antibodies. Here, a robust surface plasmon resonance-based protocol is presented, which describes a sensitive temperature-dependent kinetic measurement and evaluation method.
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