Related Experiment Video
Updated: May 21, 2026

Employing Pressurized Hot Water Extraction (PHWE) to Explore Natural Products Chemistry in the Undergraduate Laboratory
Published on: November 7, 2018
[Study on the chemical constituets in ethyl acetante extraction from semen litchi]
Kai-Wen Huang1, Jie-Wen Guo, Jian-Mei Chen
1The First People's Hospital of Shunde, Foshan 528300, China. hkwen@21cn.com
Objective:
To study the chemical constituents in ethyl acetate extraction of Semen Litchi.
Methods:
The compounds were isolated and purified by column chromatography on silica gel and Sephadex LH-20 coupled with preparative silica gel TLC, their structures were identified by physicochemical properties and spectrum analysis.
Results:
Five compounds were isolated and identified as stigmasterol (1), P-hydroxy-benzaldehyde (2), protocatechuic acid (3), daucosterol (4) and kaempferol-3-O-beta-D-glucopyranoside (5).
Conclusion:
Compounds 2 and 5 are obtained from this plant for the first time.
