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[Immunomodulatory effects of Opuntia dillenii polysaccharides on specific immune function of mice]
Long-Yan Zhao1, Song-Lian Zhang, Qing-Xia Yuan
1College of Life Science and Technology, Zhanjiang Normal University, Zhanjiang 524048, China. zhaolongyan-dra@163.com
Objective:
To investigate specific immune modulation of Opuntia dillenii polysaccharides (ODPs) on mice (body fluid and cells).
Methods:
An immunosuppressed murine model was induced by intraperitoneal injection of cyclophosphamide (Cy, ip). The intraserous hemolysin IgM, IgG levels, the proliferation of spenocytes and the proportion of T lymphocyte subsets in peripheral blood were determined after the mice treated by ODPs. Proliferative effects of ODP-I, the main component purified from ODPs, on the lymphocytes in vitro were also studied.
Results:
ODPs could significantly increase intraserous IgM and IgG levels, significantly enhance the proliferation of T and B lymphocytes and restore them to normal level. ODPs significantly reduced the percentage of CD4+ T lymphocytes subset, therefore significantly reduced the proportion of CD4+ and CD8+ T lymphocytes of peripheral blood from immunosuppressive mice and restored it to normal level. ODP-I significantly enhanced the natural proliferation of splenic lymphocyte and assisted the proliferation of T and B lymphocytes which induced by mitogen ConA or LPS.
Conclusion:
ODPs can enhance the specific immune function of immunosuppressed mice and the proliferation of lymphocytes in vitro.
