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Updated: Jan 31, 2026
11:48
Development of a Colloidal Gold-based Immunochromatographic Test Strip for Detection of Cetacean Myoglobin
Published on: July 13, 2016
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Staining Traditional Colloidal Gold Test Strips with Pt Nanoshell Enables Quantitative Point-of-Care Testing with
Di Huang1, Bingqian Lin1, Yanling Song2
1MOE Key Laboratory of Spectrochemical Analysis & Instrumentation, Collaborative Innovation Center of Chemistry for Energy Materials, Key Laboratory for Chemical Biology of Fujian Province, State Key Laboratory of Physical Chemistry of Solid Surfaces, College of Chemistry and Chemical Engineering , Xiamen University , Xiamen 361005 , China.
ACS Applied Materials & Interfaces
|December 21, 2018
Summary
Platinum nanoshells on colloidal gold test strips enable sensitive, quantitative point-of-care detection of biomarkers like myoglobin. This stable, low-cost method enhances traditional test strips for resource-limited settings.
Area of Science:
- Biomarker detection
- Nanotechnology
- Point-of-care diagnostics
Background:
- Colloidal gold immunochromatographic test strips offer portable, low-cost point-of-care (POC) testing but are typically qualitative or semi-quantitative.
- Alternative quantitative methods using magnetic or fluorescent particles require specialized instrumentation.
- Platinum nanoparticle (PtNP) assays offer quantitative results but suffer from long-term instability.
Purpose of the Study:
- To develop a stable, quantitative detection method for point-of-care testing (POCT) by enhancing colloidal gold test strips.
- To introduce platinum nanoshells onto colloidal gold surfaces for improved signal amplification and quantitative detection.
- To assess the compatibility and performance of the developed Pt-staining method using a model biomarker.
Main Methods:
- A Pt-staining method was developed to create platinum nanoshells on the surface of colloidal gold nanoparticles.
- The modified colloidal gold test strips were used for the quantitative detection of myoglobin as a model biomarker.
- The method's compatibility was demonstrated using commercially available colloidal gold test strips.
Main Results:
- The Pt-staining method successfully created stable platinum nanoshells on colloidal gold, preserving the benefits of gold nanoparticle synthesis.
- Quantitative detection of myoglobin was achieved with a limit of detection of 5.47 ng/mL in diluted serum samples.
- The Pt-staining method showed compatibility with existing commercial colloidal gold test strips.
Conclusions:
- The developed Pt-staining method enhances colloidal gold test strips for sensitive and quantitative biomarker detection.
- This approach offers a stable, low-cost, user-friendly, and compatible solution for point-of-care diagnostics.
- The technology is particularly promising for quantitative biomarker monitoring in resource-limited settings.
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