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A turn-on fluorescent sensor for imaging labile Fe(3+) in live neuronal cells at subcellular resolution
Yibin Wei1, Ziya Aydin, Yi Zhang
1Department of Chemistry and Biochemistry and UMass Cranberry Health Research Center, University of Massachusetts Dartmouth, 285 Old Westport Road, Dartmouth, MA 02747, USA.
Abstract:
An eye for an iron: A highly sensitive, selective and reversible turn-on Fe(3+) sensor for imaging labile Fe(3+) in live cells at subcellular resolution is reported. The sensor can respond to changes in intracellular Fe(3+) levels and was used to image endogenous chelatable Fe(3+) in live human neuroblastoma SH-SY5Y cells, with two Fe(3+) pools being identified in mitochondria and endosomes/ lysosomes for the first time.
