Related Experiment Video
Updated: May 21, 2026
Semi-automated Analysis of Mouse Skeletal Muscle Morphology and Fiber-type Composition
Published on: August 31, 2017
[The relationship between contractile characteristics and fiber type conversion in hind-limb unloading mice soleus]
Li Li1, Hong-Ju Liu, Ming-Hao Yang
1Institute of Basic Medical Sciences, Academy of Military Medical Sciences, Beijing 100850, China.
Objective:
To explore the relationship between contractile characteristics and fiber type conversion in hind-limb unloading mice soleus.
Methods:
After 28-day hind-limb unloading and muscle atrophy, we used the method of isolated muscle perfusion with different stimulated protocols to determine the changes in contractile characteristics including the isometric twitch force and tetanus force and fatigue index of slow twitch muscle in mice. The muscle myofibrillar composition and fiber type conversion were detected by immunofluorescence staining and real-time PCR.
Results:
The isometric twitch force and the tetanus force and fatigue index were decreased progressively in 28-day unloaded mice soleus, with the increase in fast twitch fiber subtype and the decrease in slow twitch fiber subtype.
Conclusion:
The alteration of contractile characteristics is relevant to the slow-to-fast fiber conversion in mice soleus after 28-day hind-limb unloading.
More Related Videos
08:12Intact Short, Intermediate, and Long Skeletal Muscle Fibers Obtained by Enzymatic Dissociation of Six Hindlimb Muscles of Mice: Beyond Flexor Digitorum Brevis
Published on: December 1, 2023
14:02Ex Vivo Assessment of Contractility, Fatigability and Alternans in Isolated Skeletal Muscles
Published on: November 1, 2012
