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DNA detection on lateral flow test strips: enhanced signal sensitivity using LNA-conjugated gold nanoparticles

Shiva K Rastogi1, CharLene M Gibson, Josh R Branen

  • 1Biosensors and Nanotechnology Applications Laboratory, University of Idaho, Coeur d'Alene, ID 83814-2277, USA. srastogi@uidaho.edu

Chemical Communications (Cambridge, England)
|June 30, 2012
PubMed

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Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...