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Isolation and Characterization of Dendritic Cells and Macrophages from the Mouse Intestine
Published on: May 21, 2012
[Isolation and characterization of dendritic cells from normal human esophageal mucosa]
Cun-liang Wang1, Han-bing Ning, Yan-hong Wang
1Department of Pharmacy, First Affiliated Hospital, Zhengzhou University, China.
Aim:
To investigate the characteristics of dendritic cells (DCs) from normal human esophageal mucosa.
Methods:
We collected normal esophageal mucosa samples from healthy individuals using endoscope and isolated mononuclear cells from the samples using Ficoll-Hypaque density gradient centrifugation. DCs were further separated by magnetic-activated cell sorting (MACS) to analyze DCs subsets by flow cytometry.
Results:
Three mononuclear cell populations were detected in normal human esophageal mucosa: HLA-DR(high)/CD13(low);, HLA-DR(med)/CD13(+); and HLA-DR(-)/CD13(+). HLA-DR(high)/CD13(low); cells expressed the DCs-associated molecules and were referred to as esophageal mucosal DCs. The DCs were immature since they expressed low levels of CD80, CD83 and CD86.
Conclusion:
HLA-DR(high)/CD13(low); cells isolated from the normal human esophageal mucosa were proved to be DCs. DCs can be successfully isolated from esophageal mucosa.
