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Related Concept Videos

Enzyme-Linked Immunosorbent Assay01:33

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or quantified.

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Hydrogel Nanoparticle Harvesting of Plasma or Urine for Detecting Low Abundance Proteins
10:05

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Published on: August 7, 2014

Nanoparticles rapidly assess specific IgE in plasma.

Sarmadia Ashraf1, Shahnaz Qadri, Basel al-Ramadi

  • 1Center for Research Excellence in Nanobiosciences, University of North Carolina, 321 McIver Street Greensboro, NC 27412, USA.

Nanotechnology
|July 12, 2012
PubMed
Summary

This study shows a new, fast method to detect peanut allergy indicators in plasma using magnetic nanoparticles. This point-of-care test offers comparable efficiency to the gold standard but with significantly reduced turnaround time.

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Last Updated: May 20, 2026

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10:05

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Published on: August 7, 2014

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Application of Biochip Microfluidic Technology to Detect Serum Allergen-specific Immunoglobulin E (sIgE)
07:10

Application of Biochip Microfluidic Technology to Detect Serum Allergen-specific Immunoglobulin E (sIgE)

Published on: April 21, 2019

Area of Science:

  • Immunology
  • Biotechnology
  • Medical Diagnostics

Background:

  • Allergy is a significant global chronic disease.
  • Accurate and rapid allergy diagnosis is crucial for patient management.
  • Peanut allergy is prevalent and potentially fatal, necessitating efficient diagnostic tools.

Purpose of the Study:

  • To demonstrate the feasibility of a novel point-of-care diagnostic method for allergy indicators.
  • To develop a non-invasive plasma-based test for allergy detection.
  • To compare the efficiency and speed of the new method against the established gold standard.

Main Methods:

  • Utilized magnetic nanoparticles coated with peanut protein extract.
  • Detected peanut-specific immunoglobulin E (IgE) in human plasma samples.
  • Employed a point-of-care detection procedure.

Main Results:

  • Successfully detected peanut immunoglobulin E (IgE) at concentrations near the minimum detection limit of the CAP assay.
  • Achieved diagnostic results in minutes.
  • Demonstrated comparable efficiency to the gold standard assay.

Conclusions:

  • The developed magnetic nanoparticle-based assay is a feasible, rapid, and non-invasive method for detecting allergy indicators.
  • This point-of-care approach significantly reduces diagnostic turnaround time compared to traditional methods.
  • The method shows promise for efficient allergy screening and diagnosis, particularly for conditions like peanut allergy.