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Fully Automated Centrifugal Microfluidic Device for Ultrasensitive Protein Detection from Whole Blood
08:58

Fully Automated Centrifugal Microfluidic Device for Ultrasensitive Protein Detection from Whole Blood

Published on: April 16, 2016

Blood separation on microfluidic paper-based analytical devices.

Temsiri Songjaroen1, Wijitar Dungchai, Orawon Chailapakul

  • 1Graduate Program in Clinical Biochemistry and Molecular Medicine, Faculty of Allied Health Sciences, Chulalongkorn University, Patumwan, Bangkok, 10330, Thailand.

Lab on a Chip
|July 12, 2012
PubMed
Summary
This summary is machine-generated.

This study introduces a microfluidic paper-based analytical device (μPAD) that efficiently separates blood plasma from whole blood in minutes. The device simplifies blood analysis for point-of-care diagnostics.

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Area of Science:

  • Biomedical Engineering
  • Analytical Chemistry
  • Point-of-Care Diagnostics

Background:

  • Accurate separation of blood plasma from whole blood is crucial for clinical diagnostics.
  • Existing methods can be time-consuming, require large sample volumes, and involve complex preparation steps.
  • Microfluidic paper-based analytical devices (μPADs) offer a promising platform for simplified and rapid biofluid analysis.

Purpose of the Study:

  • To develop and evaluate a novel μPAD for efficient separation of plasma from whole blood.
  • To integrate plasma separation and protein quantification into a single-step process.
  • To assess the performance of the μPAD for clinical diagnostic applications.

Main Methods:

  • Fabrication of μPADs using the wax dipping method, combining a blood separation membrane (LF1) with Whatman No.1 paper.
  • Testing of various blood separation membranes (LF1, MF1, VF1, VF2) for optimal performance.
  • Evaluation of plasma separation efficiency using human whole blood with varying hematocrit levels (24-55%).
  • Confirmation of plasma purity via microscopy to detect residual blood cells or hemolysis.
  • Quantification of plasma proteins using the bromocresol green (BCG) colorimetric assay.

Main Results:

  • The LF1 membrane proved most effective for blood separation on wax-dipped μPADs.
  • The developed LF1-μPAD successfully separated plasma from whole blood within 2 minutes for volumes of 15-22 μL.
  • Microscopy confirmed high plasma purity with no detectable blood cells or hemolysis.
  • Plasma protein detection on the μPAD showed no significant difference compared to conventional methods (p > 0.05).
  • The μPAD demonstrated good reproducibility with within-day (2.62%) and between-day (5.84%) precision.

Conclusions:

  • The developed μPAD provides an efficient and rapid method for separating blood plasma from whole blood.
  • This technology has the potential to significantly reduce turnaround time, sample volume, and preparation complexity in clinical diagnostics.
  • The μPAD is suitable for point-of-care testing, offering a simplified approach for analyzing plasma proteins.