Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Antiasthma Drugs: Mast Cell Stabilizers and Anti-IgE Drugs01:25

Antiasthma Drugs: Mast Cell Stabilizers and Anti-IgE Drugs

Asthma is a chronic respiratory condition for which new therapeutic avenues, including anti-inflammatory drugs like mast cell stabilizers and anti-IgE treatments, continue to be developed.
Mast cell stabilizers, such as cromolyn (also known as sodium cromoglycate) and nedocromil (Tilade), are effective drugs in asthma management. These stabilizers hinder histamine release by skillfully obstructing the activation of mast cells and other cellular entities. Notably, they navigate this task without...
Selectins01:25

Selectins

Cell adhesion is  an essential aspect of multicellularity. While stable cell interactions usually occur between cells of the same type, transient cell interactions occur between cells of different tissue types, such as between neutrophils and endothelial cells. Selectins are one class of cell adhesion molecules (CAMs) that bind carbohydrate ligands to form transient cell adhesion. They are rod-like proteins with a long extracellular part of variable length ending with the lectin domain, which...
Intracellular Signaling Affects Focal Adhesions01:17

Intracellular Signaling Affects Focal Adhesions

Integrins act both as extracellular input receivers and as intracellular processing activators. As their name suggests, integrins are entirely integrated into the membrane structure. Their hydrophobic membrane-spanning regions interact with the phospholipid bilayer's hydrophobic region. These membrane receptors provide extracellular attachment sites for effectors like hormones and growth factors. They activate intracellular response cascades when their effectors are bound and active.
Some...
Inflammation01:38

Inflammation

Overview
Overview of Cell-Matrix Interactions01:24

Overview of Cell-Matrix Interactions

The extracellular matrix or ECM holds cells together to form a tissue and allows the cells within the tissue to communicate. ECM comprises proteins such as fibronectin, collagen, laminin, etc. The most abundant protein in this space is collagen. Collagen fibers are interwoven with carbohydrate-containing protein molecules called proteoglycans. ECM allows cell migration and provides a structural scaffold at cell adhesion that anchors the cell when the extracellular matrix proteins interact with...
Integrins01:10

Integrins

Animal and protozoan cells do not have cell walls to help maintain shape and provide structural stability. Instead, these eukaryotic cells secrete a sticky mass of carbohydrates and proteins into the spaces between adjacent cells. This network of proteins and molecules is called an extracellular matrix or ECM.
Some ECM proteins assemble into a basement membrane to which the remaining components adhere. Proteoglycans typically form the bulk of the ECM while fibrous proteins, like collagen,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Modulation of Mast Cell Activation via MRGPRX2 by Natural Oat Extract.

International journal of molecular sciences·2026
Same author

Assessing Skin Barrier Integrity: A Comparative Study Using Transepidermal Water Loss, Electrical Impedance Spectroscopy and Corneometry.

Contact dermatitis·2026
Same author

Immunopeptidome analysis reveals SERPINB3 as an autoantigen driving eczematized psoriasis.

Science advances·2025
Same author

Beyond Fitzpatrick: automated artificial intelligence-based skin tone analysis in dermatological patients.

NPJ digital medicine·2025
Same author

Reply to Urbina, F.; Benavides, A. Telangiectatic Mastocytosis: If It Is Not Mastocytosis, What Is It? Comment on "Brockow et al. Challenges in the Diagnosis of Cutaneous Mastocytosis. <i>Diagnostics</i> 2024, <i>14</i>, 161".

Diagnostics (Basel, Switzerland)·2025
Same author

Remote evaluation of general skin diseases using three-dimensional total body photography: An observer agreement study.

Journal of the American Academy of Dermatology·2025

Related Experiment Video

Updated: May 20, 2026

Isolation of Peritoneum-derived Mast Cells and Their Functional Characterization with Ca2+-imaging and Degranulation Assays
11:31

Isolation of Peritoneum-derived Mast Cells and Their Functional Characterization with Ca2+-imaging and Degranulation Assays

Published on: July 4, 2018

Tetraspanins in mast cells.

Martin Köberle1, Susanne Kaesler, Wolfgang Kempf

  • 1Department of Dermatology, Eberhard Karls University Tübingen Tübingen, Germany.

Frontiers in Immunology
|July 12, 2012
PubMed
Summary

Tetraspanins, transmembrane proteins, are explored as novel therapeutic targets for mast cell (MC) activation. Their role in immune receptor clustering and degranulation offers potential for drug development in allergic diseases.

Keywords:
CD151CD63CD81CD9mast celltetraspanin

More Related Videos

Investigating Mast Cell Secretory Granules; from Biosynthesis to Exocytosis
16:01

Investigating Mast Cell Secretory Granules; from Biosynthesis to Exocytosis

Published on: January 26, 2015

Analyzing the Functions of Mast Cells In Vivo Using 'Mast Cell Knock-in' Mice
09:07

Analyzing the Functions of Mast Cells In Vivo Using 'Mast Cell Knock-in' Mice

Published on: May 27, 2015

Related Experiment Videos

Last Updated: May 20, 2026

Isolation of Peritoneum-derived Mast Cells and Their Functional Characterization with Ca2+-imaging and Degranulation Assays
11:31

Isolation of Peritoneum-derived Mast Cells and Their Functional Characterization with Ca2+-imaging and Degranulation Assays

Published on: July 4, 2018

Investigating Mast Cell Secretory Granules; from Biosynthesis to Exocytosis
16:01

Investigating Mast Cell Secretory Granules; from Biosynthesis to Exocytosis

Published on: January 26, 2015

Analyzing the Functions of Mast Cells In Vivo Using 'Mast Cell Knock-in' Mice
09:07

Analyzing the Functions of Mast Cells In Vivo Using 'Mast Cell Knock-in' Mice

Published on: May 27, 2015

Area of Science:

  • Immunology
  • Cell Biology
  • Pharmacology

Background:

  • Mast cells (MCs) are central immune mediators, primarily known for their role in allergic reactions.
  • MC degranulation is triggered by FcεRI-antigen-IgE complexes, but no targeted therapies currently exist.
  • Tetraspanins, transmembrane proteins, are present on MCs and act as scaffolds, influencing cellular functions.

Purpose of the Study:

  • To review the contribution of tetraspanins to mast cell functions, including FcεRI clustering and degranulation.
  • To explore the potential of tetraspanins as therapeutic targets for mast cell-mediated diseases.
  • To provide an outlook on newly described mast cell functions involving tetraspanins.

Main Methods:

  • Literature review focusing on tetraspanins in mast cell biology.
  • Analysis of tetraspanin involvement in FcεRI signaling and granule membrane organization.
  • Exploration of tetraspanin interactions with other cell surface molecules.

Main Results:

  • Tetraspanins are implicated in immune cell degranulation and receptor clustering.
  • Tetraspanins can cluster FcεRI and are found in granule membranes, influencing MC activation.
  • Tetraspanins also play roles in cell motility, adhesion, and antigen presentation.

Conclusions:

  • Tetraspanins represent promising targets for developing novel therapeutics against mast cell activation.
  • Understanding tetraspanin functions in MCs can lead to new strategies for managing allergic and inflammatory conditions.
  • Further research into tetraspanin-mediated pathways is warranted for drug development.