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Updated: May 20, 2026
Gene Regulation and Targeted Therapy in Gastric Cancer Peritoneal Metastasis: Radiological Findings from Dual Energy CT and PET/CT
Published on: January 22, 2018
siRNA interference with a proliferation-inducing ligand gene in the Sgr-7901 gastric carcinoma cell line
Shao-Zhong Ni1, Hong-Yong Cao, Zheng Chen
1Department of General Surgery, First Affiliated Hospital, Nanjing, China.
Objective:
The present study aimed to investigate the influence of siRNA interference with a proliferation- inducing ligand (APRIL) gene on gastric carcinoma sgr-7901 cell apoptosis. Correlations between APRIL silencing and tyrosine kinase (trka) expression were also explored.
Methods:
Two APRIL-silencing siRNA vectors were constructed, and transfected into human gastric carcinoma sgr-7901 cells, expression before and after transfection being detected using RT-PCR and western blot analyses. The expression of 15 trka genes was detected using RT- PCR and apoptotic rates of sgr-7901 were assessed by flow cytometry.
Results:
The expression levels of receptor trka genes were significantly decreased, and the apoptotic rate of sgr-7901 was significantly increased after transfection (P < 0.05).
Conclusion:
APRIL gene silencing can increase the apoptotic rate of gastric carcinoma cells, and inhibit the expression of receptor trka genes. There is a correlation between the signaling pathways of APRIL and trka.
Insights
APRIL gene silencing increases gastric cancer cell apoptosis and decreases tyrosine kinase receptor (trka) expression. This suggests a link between APRIL and trka signaling pathways in cancer progression.
Area of Science:
- Molecular Biology
- Oncology
- Cell Biology
Background:
- Gastric carcinoma is a significant global health concern.
- The proliferation-inducing ligand (APRIL) and tyrosine kinase receptors (trka) are implicated in cancer development.
Purpose of the Study:
- To investigate the effect of APRIL gene silencing on gastric carcinoma sgr-7901 cell apoptosis.
- To explore the correlation between APRIL silencing and trka gene expression.
Main Methods:
- APRIL-silencing siRNA vectors were transfected into sgr-7901 cells.
- Gene expression was analyzed using RT-PCR and Western blot.
- Apoptotic rates were determined by flow cytometry.
Main Results:
- APRIL gene silencing significantly increased the apoptotic rate of sgr-7901 cells (P < 0.05).
- Expression of receptor trka genes was significantly decreased post-transfection.
- A correlation was observed between APRIL silencing and trka expression levels.
Conclusions:
- APRIL gene silencing effectively enhances gastric carcinoma cell apoptosis.
- APRIL silencing inhibits the expression of receptor trka genes.
- A significant correlation exists between APRIL and trka signaling pathways in gastric cancer.
