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Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.

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Updated: May 20, 2026

Super-Resolution Imaging and Shared Management: A Protocol for Confocal Microscopy with Multiplex Detection

Super-Resolution Imaging and Shared Management: A Protocol for Confocal Microscopy with Multiplex Detection

Published on: February 24, 2026

Live-cell super-resolution imaging goes multicolor.

Teresa Klein¹, Sebastian van de Linde, Markus Sauer

¹Department of Biotechnology & Biophysics, Biozentrum, Julius-Maximilians-Universität Würzburg, Am Hubland, Würzburg, Germany.

Chembiochem : a European Journal of Chemical Biology

|July 19, 2012

Summary

This summary is machine-generated.

Researchers developed new multicolor super-resolution fluorescence imaging techniques. This breakthrough allows simultaneous imaging of over two proteins in living cells with subdiffraction resolution.

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Last Updated: May 20, 2026

Super-Resolution Imaging and Shared Management: A Protocol for Confocal Microscopy with Multiplex Detection

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Super-Resolution Live Cell Imaging of Subcellular Structures

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Published on: January 13, 2021

Area of Science:

Cell biology
Biophysics
Microscopy

Background:

Super-resolution fluorescence microscopy enables imaging beyond the diffraction limit.
Current multicolor imaging techniques often face limitations in the number of simultaneously detectable targets.

Purpose of the Study:

To expand the capabilities of multicolor super-resolution fluorescence imaging.
To enable the simultaneous visualization of more than two proteins in living cells at the nanoscale.

Main Methods:

Combined use of photoactivatable fluorescent proteins and synthetic fluorophores.
Development of novel imaging protocols for multicolor super-resolution.

Main Results:

Achieved simultaneous imaging of more than two proteins with subdiffraction optical resolution.
Demonstrated enhanced multicolor imaging capabilities in living cells.

Conclusions:

The combined approach significantly broadens options for multicolor super-resolution fluorescence imaging.
This advancement facilitates unprecedented insights into cellular processes through simultaneous multi-protein visualization.