Related Experiment Video
Updated: May 20, 2026

Reprogramming Pancreatic Ductal Adenocarcinoma to Pluripotency
Published on: February 2, 2024
Generating pluripotent stem cells: differential epigenetic changes during cellular reprogramming
1Cancer Biology and Genetics Program, Center for Cell Engineering, Sloan-Kettering Institute, Gerstner Sloan-Kettering Graduate School of Biomedical Sciences, Weill Medical College of Cornell University, 1275 York Avenue, Box #484, New York, NY 10065, United States.
Abstract:
Pluripotent stem cells hold enomous potential for therapuetic applications in tissue replacement therapy. Reprogramming somatic cells from a patient donor to generate pluripotent stem cells involves both ethical concerns inherent in the use of embryonic and oocyte-derived stem cells, as well as issues of histocompatibility. Among the various pluripotent stem cells, induced pluripotent stem cells (iPSC)--derived by ectopic expression of four reprogramming factors in donor somatic cells--are superior in terms of ethical use, histocompatibility, and derivation method. However, iPSC also show genetic and epigenetic differences that limit their differentiation potential, functionality, safety, and potential clinical utility. Here, we discuss the unique characteristics of iPSC and approaches that are being taken to overcome these limitations.
More Related Videos
10:32Cell Surface Marker Mediated Purification of iPS Cell Intermediates from a Reprogrammable Mouse Model
Published on: September 6, 2014
09:07Chemical Reversion of Conventional Human Pluripotent Stem Cells to a Naïve-like State with Improved Multilineage Differentiation Potency
Published on: June 10, 2018
Related Concept Videos
Somatic to iPS Cell Reprogramming
Chromatin Modification in iPS Cells
Compact chromatin makes reprogramming difficult. Enzymes, such as histone demethylases and acetyltransferases, are often added during reprogramming to loosen the chromatin, making the DNA more accessible to transcription factors. Molecules that inhibit histone...
Methods of Nuclear Reprogramming
Induced Pluripotent Stem Cells
Induced Pluripotent Stem Cells
Induced Pluripotent Stem Cells
Somatic cells are...