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Updated: May 20, 2026
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Development and Validation of an Ultrasensitive Single Molecule Array Digital Enzyme-linked Immunosorbent Assay for Human Interferon-α
Published on: June 14, 2018
[Production of purified human recombinant alpha2b-interferon]
Mikrobiolohichnyi Zhurnal (Kiev, Ukraine : 1993)
|July 27, 2012
Summary
Researchers developed a new method to produce biologically active recombinant human interferon alpha-2b using bacterial strains. This technology yields a highly pure and potent therapeutic protein for potential medical applications.
Area of Science:
- Biotechnology
- Molecular Biology
- Protein Production
Background:
- Recombinant human interferon alpha-2b (IFN-α2b) is a crucial therapeutic protein.
- Efficient and scalable production methods are essential for its availability.
Purpose of the Study:
- To develop a technology for producing biologically active recombinant IFN-α2b.
- To establish bacterial producer strains for large-scale synthesis.
Main Methods:
- Construction of bacterial producer strains containing human interferon alpha cDNA.
- Optimization of recombinant protein synthesis within inclusion bodies.
- Development of isolation and purification protocols.
Main Results:
- Successfully generated two bacterial producer strains for recombinant IFN-α2b.
- Achieved high purity of the drug, approximately 97-98%.
- Demonstrated significant biological activity of 4.2*10^8 IU/mg in cytopathic effect assays.
Conclusions:
- The developed technology enables efficient production of high-purity, biologically active recombinant IFN-α2b.
- The established bacterial strains and purification methods are suitable for therapeutic applications.
- This advancement contributes to the accessible supply of recombinant interferon for medical use.
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