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Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
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Updated: May 20, 2026

Microfluidic Chip Fabrication and Method to Detect Influenza
09:43

Microfluidic Chip Fabrication and Method to Detect Influenza

Published on: March 26, 2013

A high-speed, high-performance on-chip integrated reverse transcription (RT)-microchip.

Hwanyong Lee1, Nari Han, In-Hak Choi

  • 1Department of Nano Engineering, Center for Nano Manufacturing, Inje University, 607 Obang-dong, Gimhae, GyongNam, 621-749, Republic of Korea.

Biomedical Microdevices
|July 27, 2012
PubMed
Summary

This study presents a novel integrated microchip for rapid RNA extraction and complementary DNA (cDNA) synthesis from blood. The developed reverse transcription (RT)-microchip offers superior sensitivity for downstream molecular analysis.

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Last Updated: May 20, 2026

Microfluidic Chip Fabrication and Method to Detect Influenza
09:43

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Published on: March 26, 2013

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10:44

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Published on: June 20, 2018

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs
07:27

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs

Published on: August 3, 2011

Area of Science:

  • Biotechnology
  • Molecular Biology
  • Microfluidics

Background:

  • Efficient RNA extraction and complementary DNA (cDNA) synthesis are crucial for molecular diagnostics.
  • Existing methods can be time-consuming and may not offer optimal sensitivity.

Purpose of the Study:

  • To develop and validate an integrated on-chip reverse transcription (RT)-microchip for simultaneous RNA extraction and cDNA synthesis.
  • To evaluate the sensitivity of the RT-microchip compared to conventional methods.

Main Methods:

  • Development of an on-chip system integrating RNA extraction via lateral magnetophoresis with magnetic oligo-dT beads and subsequent cDNA synthesis.
  • Comparison of RT-PCR amplification efficiency using cDNA from the RT-microchip against silica column and magnetic bead methods.

Main Results:

  • Rapid RNA extraction from peripheral blood lysate in under 1 minute.
  • RT-PCR amplification showed 2-fold stronger band intensity compared to silica columns and 2.65-fold stronger than magnetic beads.
  • The RT-microchip demonstrated the highest sensitivity among the tested methods.

Conclusions:

  • The integrated RT-microchip provides a rapid and highly sensitive platform for molecular analysis.
  • This technology has potential for improving nucleic acid-based diagnostic assays.