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Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.

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Updated: May 19, 2026

Rapid Nanoprobe Signal Enhancement by In Situ Gold Nanoparticle Synthesis

Rapid Nanoprobe Signal Enhancement by In Situ Gold Nanoparticle Synthesis

Published on: March 7, 2018

Fluorescence enhancement using silver nanotriangle arrays.

Ravi Kumar Kannadorai¹, Gopalkrishna Hegde, Anand Asundi

¹School of Mechanical and Aerospace Engineering, Nanyang Technological University, 639798, Singapore.

Journal of Nanoscience and Nanotechnology

|August 3, 2012

Summary

This summary is machine-generated.

Researchers created silver nanotriangle arrays using angle-resolved nanosphere lithography to enhance fluorescence. This method achieved a tenfold increase in fluorescence intensity for fluorescein isothiocyanate, demonstrating potential for improved optical sensing applications.

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Published on: November 27, 2012

Area of Science:

Nanotechnology
Plasmonics
Optical Materials

Background:

Nanosphere lithography is a technique for creating nanoscale patterns.
Plasmon resonance in metallic nanostructures can enhance optical phenomena.
Fluorescence enhancement is crucial for sensitive detection methods.

Purpose of the Study:

To fabricate silver nanotriangle arrays using a modified nanosphere lithography technique.
To investigate the use of these arrays for enhancing fluorescence emission.
To understand the mechanism behind the observed fluorescence enhancement.

Main Methods:

Angle-resolved nanosphere lithography was employed for silver deposition.
The plasmon resonance wavelength of the silver nanotriangle array was characterized.
Fluorescence intensity of fluorescein isothiocyanate was measured on the array and on glass.

Main Results:

Silver nanotriangle arrays with controllable surface area and density were fabricated.
The plasmon resonance wavelength (470 nm) matched the fluorophore excitation wavelength.
A tenfold enhancement in fluorescence emission intensity was observed.

Conclusions:

Angle-resolved nanosphere lithography is effective for creating plasmonic nanotriangle arrays.
Silver nanotriangle arrays significantly enhance fluorescence emission.
The enhancement is attributed to localized field enhancement effects.