Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

The DNA Replication Fork01:02

The DNA Replication Fork

An organism’s genome needs to be duplicated in an efficient and error-free manner for its growth and survival. The replication fork is a Y-shaped active region where two strands of DNA are separated and replicated continuously. The coupling of DNA unzipping and complementary strand synthesis is a characteristic feature of a replication fork.   Organisms with small circular DNA, such as E. coli, often have a single origin of replication; therefore, they have only two replication forks, one in...
The DNA Replication Fork01:02

The DNA Replication Fork

An organism’s genome needs to be duplicated in an efficient and error-free manner for its growth and survival. The replication fork is a Y-shaped active region where two strands of DNA are separated and replicated continuously. The coupling of DNA unzipping and complementary strand synthesis is a characteristic feature of a replication fork.   Organisms with small circular DNA, such as E. coli, often have a single origin of replication; therefore, they have only two replication forks, one in...
The Replisome03:01

The Replisome

DNA replication is carried out by a large complex of proteins that act in a coordinated matter to achieve high-fidelity DNA replication. Together this complex is known as the DNA replication machinery or the replisome.
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with the...
The Replisome03:01

The Replisome

DNA replication is carried out by a large complex of proteins that act in a coordinated matter to achieve high-fidelity DNA replication. Together this complex is known as the DNA replication machinery or the replisome.
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with the...
Restarting Stalled Replication Forks02:37

Restarting Stalled Replication Forks

DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart, a...
Lagging Strand Synthesis01:59

Lagging Strand Synthesis

During replication, the complementary strands in double-stranded DNA are synthesized at different rates. Replication first begins on the leading strand. Replication starts later, occurs more slowly, and proceeds discontinuously on the lagging strand.
There are several major differences between synthesis of the leading strand and synthesis of the lagging strand. 1) Leading strand synthesis happens in the direction of replication fork opening, whereas lagging strand synthesis happens in the...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Polymer-Carbon Black Composites for Humidity-Driven Water Uptake and Photothermally Induced Rapid Desorption.

ACS applied materials & interfaces·2025
Same author

A Study on Thermal Conductivity Enhancement in Composites Utilizing Excluded Volume Effects.

Langmuir : the ACS journal of surfaces and colloids·2025
Same author

Development of Covalently Functionalized Alginate-Pyrrole and Polypyrrole-Alginate Nanocomposites as 3D Printable Electroconductive Bioinks.

Materials (Basel, Switzerland)·2025
Same author

Coupling between electrons' spin and proton transfer in chiral biological crystals.

Proceedings of the National Academy of Sciences of the United States of America·2025
Same author

Bistable Functions and Signaling Motifs in Systems Chemistry: Taking the Next Step Toward Synthetic Cells.

Accounts of chemical research·2025
Same author

Long-Range Proton Channels Constructed via Hierarchical Peptide Self-Assembly.

Advanced materials (Deerfield Beach, Fla.)·2024
Same journal

Dual-Function Halide Exchange Strategy for Simultaneous Sn<sup>4+</sup> Elimination and Stability Enhancement in Pb-Sn Mixed Perovskite Solar Cells.

ACS nano·2026
Same journal

Vertically Stacked Indium Gallium Zinc Oxide-Based Three-Dimensional Integrated Circuits.

ACS nano·2026
Same journal

Tunable Nanoparticle Thin-Film Reveals Distance Dependence of Auger-Mediated Radiation Enhancement in Diffuse Midline Glioma.

ACS nano·2026
Same journal

G-Quadruplex Network Engineering in Ionogels: Realizing Robust Biosensing Interfaces for Plant Electrophysiology.

ACS nano·2026
Same journal

Announcing the 2026 <i>ACS Nano</i> Lectureship and <i>ACS Nano</i> Impact Award Laureates.

ACS nano·2026
Same journal

Ultrafast Self-Assembly of Zeolitic Imidazolate Framework-8 Enables Antibody Orientation for Ultrasensitive Lateral Flow Immunoassays.

ACS nano·2026
See all related articles

Related Experiment Video

Updated: May 19, 2026

Electrophoretic Analysis of Replication Through Structure-Prone DNA Repeats Within the SV40-Based Human Episome
05:22

Electrophoretic Analysis of Replication Through Structure-Prone DNA Repeats Within the SV40-Based Human Episome

Published on: September 13, 2024

Transient fibril structures facilitating nonenzymatic self-replication.

Boris Rubinov1, Nathaniel Wagner, Maayan Matmor

  • 1Department of Chemistry, Ben Gurion University of the Negev, Beer Sheva, Israel.

ACS Nano
|August 4, 2012
PubMed
Summary
This summary is machine-generated.

Researchers developed self-synthesizing materials using amphiphilic peptides. Specific peptide fibrils act as autocatalysts, promoting peptide synthesis and self-replication, mimicking prion propagation.

More Related Videos

Visualizing Single-molecule DNA Replication with Fluorescence Microscopy
15:57

Visualizing Single-molecule DNA Replication with Fluorescence Microscopy

Published on: October 9, 2009

Self-assembly of Complex Two-dimensional Shapes from Single-stranded DNA Tiles
10:23

Self-assembly of Complex Two-dimensional Shapes from Single-stranded DNA Tiles

Published on: May 8, 2015

Related Experiment Videos

Last Updated: May 19, 2026

Electrophoretic Analysis of Replication Through Structure-Prone DNA Repeats Within the SV40-Based Human Episome
05:22

Electrophoretic Analysis of Replication Through Structure-Prone DNA Repeats Within the SV40-Based Human Episome

Published on: September 13, 2024

Visualizing Single-molecule DNA Replication with Fluorescence Microscopy
15:57

Visualizing Single-molecule DNA Replication with Fluorescence Microscopy

Published on: October 9, 2009

Self-assembly of Complex Two-dimensional Shapes from Single-stranded DNA Tiles
10:23

Self-assembly of Complex Two-dimensional Shapes from Single-stranded DNA Tiles

Published on: May 8, 2015

Area of Science:

  • Supramolecular chemistry
  • Materials science
  • Biochemistry

Background:

  • Self-synthesizing materials are a novel research area.
  • Controlling amphiphilic molecule assembly into nanostructures is key for designing these materials.
  • Amphiphilic peptides can self-assemble into various nanostructures.

Purpose of the Study:

  • To investigate the self-assembly of short amphiphilic peptides into nanostructures.
  • To explore the autocatalytic activity of these peptide nanostructures in synthesizing monomeric peptides.
  • To model and simulate the self-assembly and self-replication processes.

Main Methods:

  • Studied the self-assembly of short amphiphilic peptides into β-plates, fibrils, and nanotubes.
  • Performed kinetic analysis of self-assembly and self-replication.
  • Developed a model to simulate the replication process.
  • Investigated the mechanism of fibril reproduction.

Main Results:

  • Identified specific soluble β-sheet structures, primarily fibrils, as active autocatalysts.
  • Demonstrated that these peptide fibrils accelerate the synthesis of monomeric peptides.
  • Found that fibril stability within a few hours is crucial for catalytic activity.
  • Observed fibril reproduction via a mechanism similar to prion propagation.

Conclusions:

  • Short amphiphilic peptides can form self-synthesizing supramolecular structures.
  • Specific peptide fibrils exhibit autocatalytic activity and self-replication capabilities.
  • The findings provide insights into designing self-synthesizing materials and understanding prion-like mechanisms.