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Published on: July 17, 2012
Tomographic imaging of ratiometric fluorescence resonance energy transfer in scattering media
1Department of Biomedical Engineering, School of Medicine, Tsinghua University, Beijing, China.
Abstract:
A method to visualize and quantify fluorescence resonance energy transfer (FRET) in scattering media is proposed. It combines the ratiometric FRET method with fluorescence molecular tomography (FMT) in continuous wave (CW) mode. To evaluate the performance of the proposed method, experiments on a tissue-mimicking phantom are carried out. The results demonstrate that the proposed approach is capable of visualizing and quantifying the FRET distribution in scattering media, which implies the further application of the ratiometric assay in in vivo studies.
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