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Related Experiment Video

Updated: May 19, 2026

Analysis of Craniomaxillofacial Malformations in Mice Using Three-dimensional Microcomputed Tomography
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A novel 3D mouse embryo atlas based on micro-CT.

Michael D Wong1, Adrienne E Dorr, Johnathon R Walls

  • 1Department of Medical Biophysics, University of Toronto, and Hospital for Sick Children, Toronto, ON M5G 2M9, Canada. mwong@phenogenomics.ca

Development (Cambridge, England)
|August 9, 2012
PubMed
Summary

The International Mouse Phenotyping Consortium created a 3D mouse embryo atlas for automated analysis. This resource aids in identifying anatomical variations in mutant mouse embryos, crucial for genetic research.

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Area of Science:

  • Developmental biology
  • Genetics
  • Bioinformatics

Background:

  • The International Mouse Phenotyping Consortium (IMPC) aims to phenotype 23,000 mouse genes.
  • Embryonic lethality in knockout mice necessitates embryo-specific phenotyping methods.
  • High-throughput, automated, and quantitative methods are needed for large-scale mouse studies.

Purpose of the Study:

  • To develop automated tools for quantifying mouse embryo morphology from 3D imaging.
  • To create a representative 3D average atlas of mouse embryos for phenotypic assessment.
  • To establish baseline anatomical measurements for identifying abnormalities in mutant mouse embryos.

Main Methods:

  • Acquisition of 35 micro-CT images of C57BL/6J mouse embryos at 15.5 days post-coitum.

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  • Automated image registration software to create a consensus average 3D image.
  • Manual segmentation of 48 distinct anatomical structures within the average embryo atlas.

    • Main Results:

    • The study reports the mean and variation in volumes for 48 segmented anatomical structures.
    • Normalized mouse organ volumes exhibit a linear scale variation of 2.6-4.2%.
    • A power analysis indicates that 9-14% volume differences are detectable with a sample size of eight mice.

    Conclusions:

    • The developed 3D mouse embryo atlas provides crucial baseline anatomical data.
    • Automated registration and volume calculation will facilitate the assessment of mutant mouse phenotypes.
    • This resource is vital for the IMPC's goal of comprehensive mouse genome phenotyping.