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Related Experiment Video

Updated: May 19, 2026

Highly Sensitive and Rapid Fluorescence Detection with a Portable FRET Analyzer
08:27

Highly Sensitive and Rapid Fluorescence Detection with a Portable FRET Analyzer

Published on: October 1, 2016

A ratiometric fluorescence sensor for caffeine.

Nicolas Luisier1, Albert Ruggi, Stephan N Steinmann

  • 1Institut des Sciences et Ingénierie Chimiques, École Polytechnique Fédérale de Lausanne (EPFL), 1015 Lausanne, Switzerland.

Organic & Biomolecular Chemistry
|August 10, 2012
PubMed
Summary
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A novel molecular probe detects caffeine using fluorescence, enabling ratiometric measurements and visual coffee analysis. This method offers high selectivity for caffeine detection in aqueous solutions.

Area of Science:

  • Analytical Chemistry
  • Supramolecular Chemistry

Background:

  • Caffeine detection is crucial in food and beverage industries.
  • Development of selective and sensitive analytical probes is an ongoing challenge.

Purpose of the Study:

  • To develop a molecular probe for the fluorimetric detection of caffeine.
  • To investigate the interaction mechanism between the probe and caffeine.
  • To create a simple method for differentiating caffeinated and decaffeinated coffee.

Main Methods:

  • Synthesis and characterization of disodium 3,4:3',4'-bibenzo[b]thiophene-2,2'-disulfonate dye.
  • Fluorimetric measurements of caffeine in aqueous solutions.
  • Spectroscopic analysis to understand ground and excited state interactions.
  • Development of a test strip for visual coffee analysis.

Related Experiment Videos

Last Updated: May 19, 2026

Highly Sensitive and Rapid Fluorescence Detection with a Portable FRET Analyzer
08:27

Highly Sensitive and Rapid Fluorescence Detection with a Portable FRET Analyzer

Published on: October 1, 2016

Main Results:

  • The dye acts as a selective molecular probe for caffeine detection.
  • Non-covalent interactions in both ground and excited states lead to fluorescence response.
  • Bimodal interaction enables ratiometric measurements with high selectivity.
  • A simple test strip allows visual differentiation of normal and decaffeinated coffee using UV light.

Conclusions:

  • Disodium 3,4:3',4'-bibenzo[b]thiophene-2,2'-disulfonate is an effective probe for caffeine.
  • The probe's fluorescence response is based on specific molecular interactions.
  • The developed method is suitable for selective caffeine detection and practical coffee analysis.