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Multimodal Imaging and Spectroscopy Fiber-bundle Microendoscopy Platform for Non-invasive, In Vivo Tissue Analysis

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Dual-channel spectrally encoded endoscopic probe.

Guy Engel, Hadar Genish, Michael Rosenbluh

Biomedical Optics Express

|August 10, 2012

Summary

This study presents a novel miniature spectrally encoded endoscopy (SEE) probe for improved in vivo imaging. The new system overcomes previous limitations, offering enhanced diagnostic capabilities in hard-to-reach areas.

Keywords:

(110.2350) Fiber optics imaging (170.0170) Medical optics and biotechnology (170.2150) Endoscopic imaging (220.4610) Optical fabrication (330.1710) Color, measurement

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Area of Science:

Biomedical Engineering
Optical Imaging
Medical Diagnostics

Background:

Sub-millimeter endoscopic probes offer high-quality imaging for diagnosing hard-to-reach internal body locations.
Spectrally encoded endoscopy (SEE) shows promise but faces challenges in probe fabrication and speckle noise, hindering in vivo applications.

Purpose of the Study:

To develop and demonstrate a novel, compact, and robust miniature SEE probe.
To overcome fabrication challenges and reduce speckle noise for in vivo SEE studies.

Main Methods:

Fabrication of a high-quality miniature diffraction grating using automated femtosecond laser cutting.
Implementation of a dual-channel system with spectrally encoded imaging and separate incoherent illumination.
Optimization of imaging parameters including channel size, grating density, wavelength, and spectrum.

Main Results:

The novel SEE probe demonstrates a large depth of field and negligible back reflections.
Speckle noise is well-controlled, dependent on the illumination fiber core diameter.
Significant improvements in imaging parameters compared to previous systems were achieved.

Conclusions:

The developed miniature SEE probe represents a significant advancement in endoscopic imaging technology.
This robust system enables enhanced diagnostic capabilities in vivo, paving the way for clinical applications.