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Related Experiment Video

Updated: May 19, 2026

Microfluidic On-chip Capture-cycloaddition Reaction to Reversibly Immobilize Small Molecules or Multi-component Structures for Biosensor Applications

Microfluidic On-chip Capture-cycloaddition Reaction to Reversibly Immobilize Small Molecules or Multi-component Structures for Biosensor Applications

Published on: September 23, 2013

A serial sample loading system: interfacing multiwell plates with microfluidic devices.

Tushar D Rane¹, Helena C Zec, Tza-Huei Wang

¹Department of Biomedical Engineering, Johns Hopkins University, Baltimore, Maryland, USA.

Journal of Laboratory Automation

|August 14, 2012

Summary

This summary is machine-generated.

A new serial sample loading (SSL) system enables efficient transport of numerous samples from multiwell plates to microfluidic devices. This positive-pressure technique overcomes limitations of previous methods for high-throughput screening applications.

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Systematic Analysis of In Vitro Cell Rolling Using a Multi-well Plate Microfluidic System

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Published on: October 16, 2013

Area of Science:

Biotechnology
Microfluidics
Analytical Chemistry

Background:

High-throughput screening (HTS) demands advanced technologies for pharmaceutical and biotech industries.
Current robotic systems use microliter volumes in multiwell plates, limiting sample reduction.
Digital microfluidics reduces sample volume but lacks reliable sample transport methods.

Purpose of the Study:

To develop a novel technique for serial delivery of sample arrays to microfluidic devices.
To enable efficient sample transport from multiwell plates via a single inlet.
To address limitations in existing sample loading methods for microfluidic HTS.

Main Methods:

Development of a positive-pressure-based serial sample loading (SSL) system.
Loading serial arrays of sample plugs, separated by carrier fluid, into a capillary.
Delivery of sample plugs to a microfluidic device through a single inlet.

Main Results:

Demonstrated successful application of the SSL system for loading sample plugs into a capillary.
Showcased the system's adaptability in generating predictable sample plug volumes.
Overcame limitations of slower syringe pump or vacuum-based loading methods.

Conclusions:

The developed SSL system provides a reliable method for transporting numerous samples to microfluidic devices.
This technique facilitates reduced sample consumption in HTS.
The SSL system offers a significant advancement for microfluidic-based high-throughput screening.