Related Experiment Video

Updated: May 19, 2026

An Orthotopic Murine Model of Human Prostate Cancer Metastasis
06:48

An Orthotopic Murine Model of Human Prostate Cancer Metastasis

Published on: September 18, 2013

Genistein-induced mitotic arrest of gastric cancer cells by downregulating KIF20A, a proteomics study

Guang-Rong Yan1, Fei-Yan Zou, Bian-Li Dang

  • 1Institute of Life and Health Engineering, and National Engineering and Research Center for Genetic Medicine, Jinan University, Guangzhou, China.

Proteomics
|August 14, 2012
PubMed

Insights

Genistein, a compound with anticancer effects, works by affecting cell division. This study identifies Kinesin Family Member 20A (KIF20A) as a key protein targeted by genistein in gastric cancer cells.

Area of Science:

  • Oncology
  • Molecular Biology
  • Proteomics

Background:

  • Genistein exhibits anticancer properties by inducing cell cycle arrest and apoptosis.
  • The exact molecular mechanisms underlying genistein's anti-cancer effects remain incompletely understood.

Purpose of the Study:

  • To elucidate the molecular mechanisms of genistein's anti-cancer actions in gastric cancer.
  • To identify genistein-regulated proteins and their functional roles using quantitative proteomics.

Main Methods:

  • Quantitative proteomics was employed to analyze protein expression changes in gastric cancer cells treated with genistein.
  • Functional studies, including gene silencing and overexpression, were performed on identified key proteins.

Main Results:

  • Genistein treatment altered the expression of 86 proteins, primarily involved in cell division and G2/M transition.
  • Five kinesin family proteins, including KIF20A, were significantly downregulated by genistein.
  • Silencing KIF20A mimicked genistein's effects, inhibiting cell viability and inducing G2/M arrest, while KIF20A overexpression attenuated these effects.

Conclusions:

  • KIF20A plays a crucial role in genistein's anti-cancer activity in gastric cancer.
  • KIF20A represents a potential molecular target for gastric cancer drug development.

More Related Videos

Multi-Gene Single Nucleotide Polymorphism Detection in Gastric Cancer Based on Ion Semiconductor Sequencing Platform06:21

Multi-Gene Single Nucleotide Polymorphism Detection in Gastric Cancer Based on Ion Semiconductor Sequencing Platform

Published on: May 10, 2024

Through the Looking Glass: Time-lapse Microscopy and Longitudinal Tracking of Single Cells to Study Anti-cancer Therapeutics06:00

Through the Looking Glass: Time-lapse Microscopy and Longitudinal Tracking of Single Cells to Study Anti-cancer Therapeutics

Published on: May 14, 2016

Related Concept Videos

Mitogens and the Cell Cycle02:38

Mitogens and the Cell Cycle

Mitogens and their receptors play a crucial role in controlling the progression of the cell cycle. However, the loss of mitogenic control over cell division leads to tumor formation. Therefore, mitogens and mitogen receptors play an important role in cancer research. For instance, the epidermal growth factor (EGF) - a type of mitogen and its transmembrane receptor (EGFR), decides the fate of the cell's proliferation. When EGF binds to EGFR, a member of the ErbB family of tyrosine kinase...
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the daughter...
Inhibition of Cdk Activity02:34

Inhibition of Cdk Activity

The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
Targeted Cancer Therapies02:57

Targeted Cancer Therapies

The targeted cancer therapies, also known as “molecular targeted therapies,” take advantage of the molecular and genetic differences between the cancer cells and the normal cells. It needs a thorough understanding of the cancer cells to develop drugs that can target specific molecular aspects that drive the growth, progression, and spread of cancer cells without affecting the growth and survival of other normal cells in the body.
There are several types of targeted therapies against specific...
Drugs that Stabilize Microtubules01:15

Drugs that Stabilize Microtubules

Microtubules are dynamic structures that undergo cycles of catastrophe and rescue. The microtubules play a central role in cell division by forming the spindle apparatus for segregating the chromosomes. This makes them ideal targets for regulating dividing cells in tumors and malignant cancer cells. Microtubule stabilizing drugs help stabilize the microtubule formation and promote its polymerization. Paclitaxel was the first microtubule stabilizing agent used as anticancer drug in chemotherapy...
Negative Regulator Molecules01:23

Negative Regulator Molecules

Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.