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Related Concept Videos

Enzyme-Linked Immunosorbent Assay01:33

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
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Immunoprecipitation, or IP, is a widely used technique that employs protein-antibody interactions to isolate proteins or protein complexes in their native state for studying protein-protein interactions, quaternary structures, or supramolecular complexes. Various modifications of the technique, including chromatin IP, cross-linking IP, and fluorescence IP, are commonly used.
Chromatin Immunoprecipitation
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Development of a Lateral Flow Immunochromatographic Strip for Rapid and Quantitative Detection of Small Molecule Compounds
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Published on: November 13, 2021

Immunochromatographic assay on thread.

Gina Zhou1, Xun Mao, David Juncker

  • 1Biomedical Engineering Department, McGill University, Montreal, QC, Canada.

Analytical Chemistry
|August 15, 2012
PubMed
Summary
This summary is machine-generated.

A novel immunochromatographic assay on thread (ICAT) offers a low-cost, sensitive, and multiplexed diagnostic test. This thread-based assay provides quantitative results for point-of-care screening, overcoming limitations of traditional lateral flow assays.

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Area of Science:

  • Biomedical Engineering
  • Assay Development
  • Point-of-Care Diagnostics

Background:

  • Lateral-flow immunochromatographic assays are common for rapid, low-cost screening but typically lack quantification and multiplexing capabilities.
  • Existing thread-based liquid transport methods have not been validated for quantitative, high-sensitivity immunoassays.
  • There is a need for improved diagnostic platforms that are sensitive, quantitative, and capable of multiplexed detection.

Purpose of the Study:

  • To introduce and validate the immunochromatographic assay on thread (ICAT) as a quantitative and multiplexed diagnostic platform.
  • To demonstrate the feasibility of using threads as a support matrix for high-sensitivity immunoassays.
  • To compare the performance of ICAT with conventional paper-based and other diagnostic assays.

Main Methods:

  • Developed a cartridge-based immunochromatographic assay on thread (ICAT) using antibody-coated cotton threads and nylon fibers.
  • Optimized assay conditions and established binding curves for C-reactive protein (CRP) in buffer and diluted serum.
  • Demonstrated multiplexing by coating threads with antibodies against CRP, osteopontin, and leptin, and quantified results using a flatbed scanner.

Main Results:

  • Achieved a limit of detection of 377 pM for CRP using the ICAT platform.
  • Successfully demonstrated multiplexed detection of three different protein analytes (CRP, osteopontin, leptin).
  • ICAT showed comparable or improved performance to traditional paper-based and conventional diagnostic assays.

Conclusions:

  • Thread is a suitable and versatile support material for developing low-cost, sensitive, and user-friendly diagnostic tests.
  • The ICAT platform overcomes the limitations of traditional lateral flow assays by enabling quantitative and multiplexed detection.
  • ICAT holds significant potential for advancing point-of-care diagnostics and screening.