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A novel CRISPR/Cas14a-synergized wood-based platform for the ultrasensitive detection of aflatoxin B1
Huixin Chen1, Qing Li1, Xun Mao1
1College of Chemistry and Chemical Engineering, Central South University of Forestry and Technology, Changsha, 410004, P.R. China. xunmao@nwu.edu.cn.
Abstract:
A CRISPR/Cas14a-synergized wood-based platform (CWP) is designed here to detect aflatoxin B1 (AFB1) at an LOD of 0.67 fmol L-1 with high sensitivity and no preamplification. Once the assay is performed, AFB1 is optically identified through an aptamer competition process, which triggers the trans-cleavage activity of Cas14a, resulting in significant color changes on the wood surface upon introducing chromogenic substrates. This method can be conveniently extended to the detection of disease-related protein biomarkers with low detection thresholds.
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