Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: May 19, 2026

Derivation of Hematopoietic Stem Cells from Murine Embryonic Stem Cells
22:06

Derivation of Hematopoietic Stem Cells from Murine Embryonic Stem Cells

Published on: February 25, 2007

Combinatorial stem cell mobilization in animal models.

Simon C Pitchford1, Sara M Rankin

  • 1Leukocyte Biology Section, Faculty of Medicine National Heart and Lung Institute, Imperial College London, London, UK.

Methods in Molecular Biology (Clifton, N.J.)
|August 15, 2012
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The Platelet/Megakaryocyte Axis is Necessary for Allergic Sensitisation and Inflammatory Responses to House Dust Mite in the Lung.

Lung·2026
Same author

Platelet-specific P2Y<sub>1</sub> receptor deficient mice have suppressed pulmonary leukocyte recruitment in response to lipopolysaccharide.

Respiratory research·2026
Same author

SiO<sub>2</sub>-CaO<sub>CME</sub>/Poly(Tetrahydrofuran)/Poly(Caprolactone) 3D-Printed Scaffolds Drive Human-Bone Marrow Stromal Cell Osteogenic Differentiation.

Advanced healthcare materials·2026
Same author

Antiplatelet Therapy.

Handbook of experimental pharmacology·2026
Same author

Pseudomonas aeruginosa infection exacerbates elastase induced lung damage: characterisation of a novel murine two-hit model of pulmonary infection.

Respiratory research·2025
Same author

3D printable PCL-<i>b</i>-P(MMA-<i>co</i>-TMSPMA)/silica hybrids using a PCL RAFT agent.

Journal of materials chemistry. B·2025
Same journal

Tracking Synthetic Adhesins on Bacterial Surfaces with Immunofluorescence Microscopy.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Post-Selection Methods for Analyzing mRNA Display Selections and Optimization of Hits.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

High-Performance Computing in Tandem Mass Spectrometry (MS/MS) Peptide Identification.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Engineering and Adapting Disulfide-Containing Proteins to Enable Intracellular Functionality.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

AI-Driven Protein Research: From Prediction to Design.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Methods for the In Vitro Selection of Protein and Peptide Libraries Using mRNA Display.

Methods in molecular biology (Clifton, N.J.)·2026
See all related articles

Combining therapies can improve hematopoietic progenitor cell (HPC) mobilization for transplants. This study introduces a new method to simultaneously track HPCs, endothelial progenitor cells (EPCs), and mesenchymal stem cells (MSCs) for regenerative medicine.

Area of Science:

  • Stem cell biology
  • Regenerative medicine
  • Hematopoiesis

Background:

  • Single therapies like G-CSF have limited capacity to mobilize hematopoietic progenitor cells (HPCs) for bone marrow transplantation, leading to insufficient cell numbers in ~20% of patients.
  • Combinatorial therapies, such as G-CSF with CXCR4 antagonists, show synergistic HPC mobilization, indicating potential therapeutic benefits.
  • Endothelial progenitor cells (EPCs) and mesenchymal stem cells (MSCs) also reside in bone marrow and are crucial for tissue regeneration, but their mobilization is less understood.

Purpose of the Study:

  • To develop and validate a novel in situ perfusion system for the mouse hind limb to directly quantify stem and progenitor cell egress from the bone marrow.
  • To simultaneously assess the mobilization of hematopoietic progenitor cells (HPCs), endothelial progenitor cells (EPCs), and mesenchymal stem cells (MSCs).

More Related Videos

In vivo Clonal Tracking of Hematopoietic Stem and Progenitor Cells Marked by Five Fluorescent Proteins using Confocal and Multiphoton Microscopy
17:08

In vivo Clonal Tracking of Hematopoietic Stem and Progenitor Cells Marked by Five Fluorescent Proteins using Confocal and Multiphoton Microscopy

Published on: August 6, 2014

Related Experiment Videos

Last Updated: May 19, 2026

Derivation of Hematopoietic Stem Cells from Murine Embryonic Stem Cells
22:06

Derivation of Hematopoietic Stem Cells from Murine Embryonic Stem Cells

Published on: February 25, 2007

In vivo Clonal Tracking of Hematopoietic Stem and Progenitor Cells Marked by Five Fluorescent Proteins using Confocal and Multiphoton Microscopy
17:08

In vivo Clonal Tracking of Hematopoietic Stem and Progenitor Cells Marked by Five Fluorescent Proteins using Confocal and Multiphoton Microscopy

Published on: August 6, 2014

  • To explore the potential of combinatorial therapies for differential mobilization of these stem cell subsets for therapeutic applications.

    • Main Methods:

    • Development of an in situ perfusion system for the mouse hind limb.
    • Quantification of mobilized progenitor cells using colony-forming assays and immunohistochemistry.
    • Simultaneous measurement of hematopoietic progenitor cells (HPCs), endothelial progenitor cells (EPCs), and mesenchymal stem cells (MSCs) egress.

    Main Results:

    • The methodology allows for direct and simultaneous quantification of HPC, EPC, and MSC mobilization from the bone marrow.
    • Demonstrated that discrete combination therapies can achieve differential mobilization of these distinct stem cell subsets.
    • The system provides a robust platform for evaluating novel mobilization strategies.

    Conclusions:

    • The described in situ perfusion system is effective for simultaneously quantifying the mobilization of HPCs, EPCs, and MSCs.
    • Combinatorial therapeutic approaches can differentially mobilize specific stem cell populations.
    • Identification of novel pharmacological regimens for selective EPC and MSC mobilization holds promise for future tissue regeneration strategies.