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Monoclonal antibody selectively recognizing murine but not human CD44
Yumi Kinugasa1, Nobuyuki Takakura
1Department of Signal Transduction, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka, Japan.
Hybridoma (2005)
|August 17, 2012
Summary
Researchers developed a new monoclonal antibody, MS44, that specifically targets mouse CD44 but not human CD44. This antibody is a valuable tool for studying host CD44 in mouse models of human cancer.
Area of Science:
- Immunology
- Cancer Biology
- Biotechnology
Background:
- CD44 is a cell surface glycoprotein involved in cell adhesion, migration, and cancer progression.
- Distinguishing between host and cancer cell CD44 is crucial in preclinical cancer models.
Purpose of the Study:
- To generate a monoclonal antibody (MAb) that specifically recognizes murine CD44.
- To develop a tool for analyzing host CD44 in human cancer xenograft models.
Main Methods:
- Immunization of mice with cancer-associated fibroblasts.
- Generation and characterization of monoclonal antibody MS44.
- Immunoblotting and flow cytometry to assess antibody specificity.
Main Results:
- Monoclonal antibody MS44 was successfully generated.
- MAb MS44 specifically recognizes murine CD44, confirmed by immunoblotting.
- MAb MS44 does not react with human CD44 in flow cytometry.
Conclusions:
- MAb MS44 is a specific and effective reagent for detecting mouse CD44.
- This antibody enables the study of host CD44 in human cancer xenograft models.
- MS44 facilitates research into the role of host CD44 in cancer progression.
