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Updated: May 19, 2026

Measuring Spatial and Temporal Ca2+ Signals in Arabidopsis Plants
Published on: September 2, 2014
Cytosolic Ca(2+) determinations in studying plant responses to salinity and oxidative stress
Anuphon Laohavisit1, Renato Colaço, Julia Davies
1Department of Plant Sciences, University of Cambridge, Cambridge, UK.
Abstract:
Salinity and oxidative stress can transiently elevate cytosolic free Ca(2+) ([Ca(2+)](cyt)) of plant cells. The [Ca(2+)](cyt) increase may be part of a signaling cascade or cell death, depending on cell type, the magnitude and the duration of stress exposure. Several approaches for determining [Ca(2+)](cyt) responses are available to plant biologists, but some require highly specialized equipment. Here we describe protocols for using aequorin as a standard [Ca(2+)](cyt) reporter, with output detected with a plate-reader luminometer.
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