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Overview Of Cell Separation And Isolation

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Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.

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Updated: May 19, 2026

Technique for Obtaining Mesenchymal Stem Cell from Adipose Tissue and Stromal Vascular Fraction Characterization in Long-Term Cryopreservation

Technique for Obtaining Mesenchymal Stem Cell from Adipose Tissue and Stromal Vascular Fraction Characterization in Long-Term Cryopreservation

Published on: December 30, 2021

Efficient stem cell isolation from under vacuum preserved tissue samples.

Aldo Moggio¹, Giuseppe D'Armento, Benedetta Bussolati

¹Department of Internal Medicine, Center for Molecular Biotechnology, Torino, Italy.

|August 18, 2012

Summary

This summary is machine-generated.

Vacuum sealing and cooling effectively preserve human renal progenitor cells from surgical tissue. This method allows for practical isolation of stem/progenitor cells comparable to fresh tissue, offering a feasible alternative for clinical applications.

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Area of Science:

Regenerative Medicine
Cell Biology
Surgical Pathology

Background:

Isolation of stem/progenitor cells is crucial for regenerative medicine.
Current methods often require stringent culture conditions.
Formalin fixation is a common but potentially damaging preservation method.

Purpose of the Study:

To evaluate vacuum sealing and cooling as a method for preserving human renal progenitor cells.
To assess the feasibility of isolating viable stem/progenitor cells from preserved tissue.
To compare the characteristics of cells isolated from preserved versus fresh tissue.

Main Methods:

Human renal tissue samples (n=20) were preserved under vacuum and cooling (4°C) for 6-48 hours.
CD133(+) progenitor cells were isolated from disaggregated preserved tissues.
Phenotypic characterization, in vitro, and in vivo differentiation assays were performed.

Main Results:

Viable human renal CD133(+) progenitor cells were successfully isolated from vacuum-sealed and cooled specimens.
Isolated cells exhibited comparable phenotypic characteristics and differentiation potential to those from fresh tissue.
The preservation method yielded hypoxia-resistant stem/progenitor cells.

Conclusions:

Vacuum sealing and cooling is a suitable method for preserving human stem/progenitor cells.
This technique offers a practical and feasible approach for stem cell extraction from clinical samples.
The method supports the isolation of hypoxia-resistant progenitor cells for research and therapeutic applications.