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An Automated Radiosynthesis of [68Ga]Ga-FAPI-46 for Routine Clinical Use
Published on: May 24, 2024
(67)Ga and (68)Ga purification studies: preliminary results
R F Costa1, M F Barboza, J A Osso
1Instituto de Pesquisas Energéticas e Nucleares, Cidade Universitária, São Paulo, Brazil. renatafcosta@usp.br
Summary
Purifying Gallium-68 (68Ga) from generator eluates is crucial for radiolabeling biomolecules in positron emission tomography. Two handmade purification methods were evaluated, with extraction chromatography showing promise for metallic impurity removal.
Area of Science:
- Nuclear medicine
- Radiochemistry
- Medical imaging
Background:
- Positron emission tomography (PET) is vital for disease diagnosis.
- (68)Gallium (68Ga) is a key positron emitter for PET imaging.
- Commercial (68)Ge/(68)Ga generators yield eluates with metallic impurities that hinder biomolecular labeling.
Purpose of the Study:
- To develop and evaluate handmade purification methods for (68)Ga and (67)Ga.
- To remove metallic impurities from generator-produced (68)Ga for radiolabeling.
- To compare the efficacy of cation exchange resin and solvent extraction chromatography.
Main Methods:
- Two purification techniques were employed: cation exchange resin elution with acetone/acid and column chromatography solvent extraction.
- The cation exchange resin used was AG50W-X8 (>400 mesh).
- Solvent extraction involved diisopropyl ether absorbed in XAD-16, with (68)Ga recovered in deionized water.
Main Results:
- Cationic resin AG50W-X8 provided the best purification results, though the resin is not commercially available.
- Extraction chromatography using XAD-16 demonstrated significant promise for (68)Ga purification.
- (68)Ga recovery and purification levels were lower with the cation exchange resin method, but it offered elution in deionized water.
Conclusions:
- Handmade purification of (68)Ga is necessary to remove metallic impurities from generator eluates.
- Extraction chromatography presents a promising method for (68)Ga purification for radiolabeling applications.
- While cation exchange resin offered advantages in elution medium, extraction chromatography is highlighted as the most promising purification strategy.
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