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Updated: May 19, 2026

Protocol for Three-dimensional Confocal Morphometric Analysis of Astrocytes
Published on: December 11, 2015
Chemically functionalized water-soluble single-walled carbon nanotubes modulate morpho-functional characteristics of
Manoj K Gottipati1, Irina Kalinina, Elena Bekyarova
1Department of Neurobiology, University of Alabama, Birmingham, Alabama 35294, United States.
Abstract:
We report the use of chemically functionalized water-soluble single-walled carbon nanotubes (ws-SWCNTs) for the modulation of morpho-functional characteristics of astrocytes. When added to the culturing medium, ws-SWCNTs were able to make astrocytes larger and stellate/mature, changes associated with the increase in glial fibrillary acidic protein immunoreactivity. Thus, ws-SWCNTs could have more beneficial effects at the injury site than previously thought; by affecting astrocytes, they could provide for a more comprehensive re-establishment of the brain computational power.
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