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Isolation and Preparation of Bacterial Cell Walls for Compositional Analysis by Ultra Performance Liquid Chromatography
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Novel teichulosonic acid from cell wall of Streptomyces coelicolor M145

Alexander S Shashkov1, Bohdan E Ostash, Victor A Fedorenko

  • 1N D Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow 119991, Russian Federation.

Carbohydrate Research
|August 29, 2012
PubMed

Insights

The cell wall of Streptomyces coelicolor M145 contains novel glycopolymer structures, including a unique Kdn-containing teichulosonic acid and a poly(diglycosyl 1-phosphate). These findings advance our understanding of bacterial cell wall composition.

Area of Science:

  • Microbiology
  • Biochemistry
  • Glycobiology

Background:

  • The cell wall of Streptomyces coelicolor is a complex structure crucial for bacterial survival.
  • Previous studies have characterized some cell wall components, but novel polymers may exist.

Purpose of the Study:

  • To elucidate the structures of the main and minor glycopolymers in the cell wall of Streptomyces coelicolor M145.
  • To characterize an unusual Kdn-containing teichulosonic acid and a novel poly(diglycosyl 1-phosphate) structure.

Main Methods:

  • Chemical analysis of the cell wall components.
  • Nuclear Magnetic Resonance (NMR) spectroscopy for structural elucidation.
  • Analysis of a prototrophic plasmidless variant (SCP1(-) SCP2(-)) of S. coelicolor A3(2).

Main Results:

  • Identified Kdn-containing teichulosonic acid as the main cell wall glycopolymer with a previously undescribed structure.
  • Characterized the minor polymer as a poly(diglycosyl 1-phosphate) with a repeating unit of -6)-α-Galp-(1→6)-α-GlcpNAc-(1-P-.
  • Confirmed the structures using a combination of chemical and NMR spectroscopic techniques.

Conclusions:

  • The cell wall of Streptomyces coelicolor M145 possesses unique glycopolymer structures.
  • The identified structures contribute to the known diversity of bacterial cell wall glycopolymers.
  • This research provides a detailed molecular understanding of Streptomyces coelicolor M145 cell wall composition.

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