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Introducing the Forensic Research/Reference on Genetics knowledge base, FROG-kb.

Haseena Rajeevan1, Usha Soundararajan, Andrew J Pakstis

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FROG-kb is a new open-access web application for forensic genetics research and teaching. It utilizes single nucleotide polymorphisms (SNPs) for human identification and ancestry inference, addressing limitations of older short tandem repeat polymorphism (STRP) systems.

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Area of Science:

  • Forensic Genetics
  • Bioinformatics
  • Human Identification

Background:

  • Traditional short tandem repeat polymorphisms (STRPs) have limited discriminatory power for ethnicity due to low Fst values and common alleles.
  • Existing online tools for forensic research using genetic markers are limited.
  • Single nucleotide polymorphisms (SNPs) are increasingly researched for human identification and description.

Purpose of the Study:

  • To present FROG-kb, an open-access web application for forensic genetics.
  • To provide a tool for teaching, research, and practice in forensic investigations.
  • To offer computational tools for analyzing allele frequencies and genetic data.

Main Methods:

  • Developed FROG-kb using Oracle 10g DBMS, Java, Java Servlet, JSP, JQuery, and GoogleCharts.
  • Integrated data from the ALlele FREquency Database (ALFRED).
  • Implemented computational tools for individual identification, ancestry inference, and phenotype prediction (eye color).

Main Results:

  • Launched FROG-kb (http://frog.med.yale.edu), an open-access web application for forensic genetics.
  • FROG-kb utilizes allele frequencies from ALFRED for various analyses.
  • The tool supports individual identification, ancestry inference, and eye color prediction based on SNP panels.

Conclusions:

  • FROG-kb offers novel and useful functionalities for the forensic community.
  • The application is designed for ongoing improvement and expansion based on user input.
  • FROG-kb serves as a valuable resource for forensic research, teaching, and practice.