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Updated: May 18, 2026

Fluorescent End-Labeling and Encapsulation of Long RNAs for Single-Molecule FRET-TIRF Microscopy
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Fluorescent End-Labeling and Encapsulation of Long RNAs for Single-Molecule FRET-TIRF Microscopy

Published on: October 18, 2024

Labeling DNA (or RNA) for single-molecule FRET

Chirlmin Joo, Taekjip Ha

    Cold Spring Harbor Protocols
    |September 6, 2012
    PubMed
    Summary

    This study details a simplified protocol for labeling nucleic acids for single-molecule Förster resonance energy transfer (smFRET) using total internal reflection microscopy. This method enables precise distance measurements in biological studies.

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