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Related Experiment Videos

Intercellular communication is cell cycle modulated during early Xenopus laevis development.

J W Su1, L G Tertoolen, S W de Laat

  • 1Hubrecht Laboratory, Netherlands Institute for Developmental Biology, Utrecht.

The Journal of Cell Biology
|January 1, 1990
PubMed
Summary

Intercellular communication in Xenopus laevis development is cell cycle modulated. Gap junction abundance changes significantly during the tenth cell cycle, impacting dye coupling.

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Area of Science:

  • Developmental Biology
  • Cell Biology
  • Xenopus laevis research

Background:

  • Intercellular communication is crucial for embryonic development.
  • Understanding cell cycle regulation of communication pathways is essential.

Purpose of the Study:

  • To investigate cell cycle modulation of intercellular communication in Xenopus laevis.
  • To analyze gap junction and cytoplasmic bridge-mediated dye coupling.
  • To determine the structural basis for cell cycle-dependent changes in gap junction coupling.

Main Methods:

  • Microinjection of tracer dyes (Lucifer yellow, FITC-dextran).
  • Freeze-fracture electron microscopy to visualize gap junctions.
  • Quantification of gap junction abundance across cell cycles.

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Main Results:

  • Dye coupling via gap junctions and cytoplasmic bridges is cell cycle modulated.
  • Gap junction abundance significantly increases (sixfold) from the beginning to the end of the tenth cell cycle.
  • Morphological changes in gap junctions correlate with observed cell cycle modulation.

Conclusions:

  • Gap junction-mediated communication in Xenopus embryos is dynamically regulated by the cell cycle.
  • Changes in gap junction number are a primary mechanism underlying cell cycle-modulated intercellular communication.
  • These findings provide insights into the precise control of developmental processes through intercellular signaling.