Related Experiment Video
Updated: May 18, 2026
09:44
High-throughput Screening for Chemical Modulators of Post-transcriptionally Regulated Genes
Published on: March 3, 2015
Transcriptional biomarkers--high throughput screening, quantitative verification, and bioinformatical validation
Irmgard Riedmaier1, Michael W Pfaffl
1Technische University Munich, Weihenstephaner Berg 3, 85354 Freising, Germany. irmgard.riedmaier@wzw.tum.de
Methods (San Diego, Calif.)
|September 13, 2012
Summary
Molecular biomarkers are measurable molecules used in diagnostics and disease prognosis. This review details various molecular levels, including the transcriptome, for identifying and validating these crucial biological indicators.
Area of Science:
- Biomarker research
- Molecular medicine
- Genomics and transcriptomics
Background:
- Biomarkers are measurable biological molecules indicating physiological status, disease, or drug effects.
- They are vital tools in molecular medicine, diagnostics, prognosis, and risk assessment.
- Existing examples demonstrate the clinical utility of molecular biomarkers.
Purpose of the Study:
- To describe diverse molecular levels for biomarker research.
- To highlight biomarker candidates identified across these levels.
- To detail the transcriptomic approach for biomarker discovery.
Main Methods:
- Utilizing "omic" technologies, particularly high-throughput methods.
- Identifying and quantitatively measuring biomarkers at genomic, epigenomic, transcriptomic, proteomic, metabolomic, and lipidomic levels.
- Focusing on transcriptomic methods, including verification and biostatistical requirements.
Main Results:
- Biomarkers can be identified across multiple molecular layers.
- High-throughput "omic" technologies enable comprehensive biomarker discovery.
- The transcriptomic approach offers detailed insights into transcriptional biomarkers.
Conclusions:
- Molecular biomarkers are essential across various scientific and medical fields.
- Diverse "omic" strategies facilitate biomarker identification and measurement.
- Transcriptomic analysis provides a robust framework for biomarker validation.
Related Concept Videos
02:34DNA Microarrays
Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
02:24Ribosome Profiling
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...