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Enzyme-Linked Immunosorbent Assay

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Related Experiment Video

Updated: May 18, 2026

Snap Chip for Cross-reactivity-free and Spotter-free Multiplexed Sandwich Immunoassays

Snap Chip for Cross-reactivity-free and Spotter-free Multiplexed Sandwich Immunoassays

Published on: November 13, 2017

Chip-based immunoassays.

Akwasi A Apori¹, Amy E Herr

¹Department of Bioengineering, University of California Berkeley, Berkeley, CA, USA.

Methods in Molecular Biology (Clifton, N.J.)

|September 15, 2012

Summary

This summary is machine-generated.

This study presents a novel microfluidic device for rapid protein analysis using on-chip immunosubtraction. This technique efficiently separates bound and unbound proteins, enabling accurate determination of protein mobility and binding specificity in complex samples.

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Published on: June 23, 2022

Area of Science:

Biochemistry
Analytical Chemistry
Microfluidics

Background:

Microfluidic immunoassays offer advantages in speed, automation, and portability compared to traditional methods.
On-chip immunosubtraction is a homogeneous immunoassay for assessing protein mobility and binding specificity.

Purpose of the Study:

To describe the fabrication of a microfluidic device for on-chip immunosubtraction.
To present an electrophoretic assay protocol for determining target protein mobility and binding specificity in complex biological samples.

Main Methods:

Fabrication of patterned polyacrylamide (PA) gel regions on-chip to define sample preparation and immunosubtraction zones.
Utilizing a size-based exclusion filter to separate antibody-bound target proteins from unbound nontarget proteins.
Implementing electrophoretic detection for downstream analysis of unbound proteins.

Main Results:

Successful fabrication of the microfluidic immunosubtraction device.
Demonstration of on-chip sample preparation including protein enrichment, fluorescent labeling, and antibody-target binding.
Validation of the assay protocol for determining protein mobility and binding specificity.

Conclusions:

The developed microfluidic immunosubtraction device and assay protocol provide a rapid and efficient method for analyzing protein characteristics.
This technique is applicable to complex biological samples like cerebrospinal fluid, offering a portable alternative to bench-top methods.