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Related Experiment Videos

A cell-based approach to the human proteome project.

Neil L Kelleher1

  • 1Department of Chemistry, Northwestern University, Evanston, IL 60208, USA. n-kelleher@northwestern.edu

Journal of the American Society for Mass Spectrometry
|September 15, 2012
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

Proteomics01:33

Proteomics

9.2K
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
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The Human Proteome Project is being redefined to focus on cell-specific protein structures. This cell-based approach aims to map human cell types and their proteomes, addressing knowledge gaps in cell heterogeneity and protein isoforms.

Area of Science:

  • Proteomics
  • Cell Biology
  • Human Physiology

Background:

  • The Human Genome Project mapped human genes, but understanding protein expression within specific cell types remains a challenge.
  • Defining the proteome requires context, as protein composition varies significantly between different human cells.
  • Existing knowledge gaps exist in understanding cellular heterogeneity and the diversity of protein isoforms.

Purpose of the Study:

  • To propose a cell-based Human Proteome Project (CB-HPP) analogous to the Human Genome Project.
  • To define clear milestones and deliverables for a cell-centric proteomic initiative.
  • To highlight the need for technological advancements in cell type definition and proteome analysis.

Main Methods:

  • Focusing on protein primary structure as expressed in specific cell types.

Related Experiment Videos

  • Developing a framework for mapping and sorting diverse human cell types.
  • Utilizing advanced protein mass spectrometry techniques beyond the current state-of-the-art.

    • Main Results:

    • The CB-HPP concept provides a structured approach to proteomic research.
    • Identifies critical knowledge gaps concerning cell heterogeneity and protein isoforms.
    • Establishes the necessity for new technologies to comprehensively analyze cell-specific proteomes.

    Conclusions:

    • A cell-based Human Proteome Project is essential for a complete understanding of human biology.
    • Addressing cell heterogeneity and protein isoforms requires innovative technological solutions.
    • The CB-HPP will guide future research in defining and characterizing all human cell proteomes.