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Updated: Feb 21, 2026

Isolation of Preadipocytes from Broiler Chick Embryos
Published on: August 4, 2022
[Effect of perilipin 1 on chicken preadipocyte lipid accumulation]
Wei-nan Zhou1, Yu-xiang Wang, Hui Li
1College of Animal Science and Technology, Northeast Agricultural University, Harbin, China. zwn0501@163.com
Aim:
To explore the effect of perilipin 1 on chicken preadipocyte lipid accumulation.
Methods:
Firstly, pcDNA3.1-perilipin 1 gene eukaryotic expression vector was constructed and transfected into primary cultured chicken preadipocytes. Twenty-four hours after transfection, chicken preadipocytes were cultured in complete medium with oleate, to induce preadipocyte differentiation. Then perilipin 1 was detected by Western blotting; the role of perilipin 1 in preadipocyte lipid accumulation was investigated by Oil Red O staining extraction assay; the expression levels of other genes (FAS, ACC and ATGL) related to the chicken adipocyte lipid metabolism were tested by real-time RT-PCR.
Results:
pcDNA3.1-perilipin 1 gene eukaryotic expression vector upregulated the expression of perilipin 1 in the transfected chicken preadipocytes. Over-expression of perilipin 1 promoted chicken preadipocyte lipid accumulation, while there was no significant change in the expression levels of other chicken lipid metabolism related genes (FAS, ACC and ATGL).
Conclusion:
Over-expression of perilipin 1 can promote chicken preadipocyte lipid accumulation.
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