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Updated: Jan 25, 2026
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Identification and Isolation of Oligopotent and Lineage-committed Myeloid Progenitors from Mouse Bone Marrow
Published on: July 29, 2018
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Developmental fate and lineage commitment of singled mouse blastomeres
Chanchao Lorthongpanich1, Tham Puay Yoke Doris, Vachiranee Limviphuvadh
1Mammalian Development Laboratory, Institute of Medical Biology, 8A Biomedical Grove, #06-06 Immunos, Singapore 138648. Chanchao.lorthongpanich@imb.a-star.edu.sg
Development (Cambridge, England)
|September 20, 2012
Summary
Cell-cell interactions and position are crucial for mammalian embryo development. Separating blastomeres reveals they tend towards trophectoderm fate without these cues.
Area of Science:
- Developmental Biology
- Cell Biology
- Genetics
Background:
- The inside-outside model explains early mammalian embryo cell-fate specification.
- Cell-cell interactions and positional information are thought to be key factors.
Purpose of the Study:
- To investigate the influence of cell-cell interactions and positional information on blastomere fate specification.
- To determine the developmental potential of blastomeres isolated from normal embryonic interactions.
Main Methods:
- Sequential separation of blastomeres from two-cell stage mouse embryos throughout pre-implantation development.
- Analysis of gene expression profiles, polarity protein localization, and functional contribution to lineages.
Main Results:
- Separated blastomeres, lacking cell contact and positional cues, exhibit a unique gene expression pattern.
- These isolated blastomeres show a tendency towards a trophectoderm-like gene expression profile.
- Functional tests confirm a preferential contribution of isolated blastomeres to the trophectoderm lineage.
Conclusions:
- Cell-cell interactions and position significantly influence blastomere fate specification in mammalian embryos.
- Absence of these cues leads to a default trophectoderm-like developmental trajectory.
- Positional information plays a critical role in establishing distinct cell lineages during early embryogenesis.
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