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A miniaturized method for estimating di(2-ethylhexyl) phthalate in bivalves as bioindicators
M Muñoz-Ortuño1, Y Moliner-Martínez, S Cogollos-Costa
1Departamento Química Analítica, Facultad de Química, Universidad de Valencia, C/Dr Moliner, 50-46100 Burjassot, Valencia, Spain.
Journal of Chromatography. A
|September 22, 2012
Summary
A new, rapid method analyzes di(2-ethylhexyl) phthalate (DEHP) in aquatic organisms using matrix solid phase dispersion (MSPD). This technique offers efficient DEHP detection in mussels and coastal waters, aiding environmental monitoring.
Area of Science:
- Environmental Chemistry
- Analytical Chemistry
- Marine Biology
Background:
- Di(2-ethylhexyl) phthalate (DEHP) is a common environmental contaminant.
- Phthalates can bioaccumulate in aquatic organisms, posing ecological risks.
- Accurate quantification of DEHP in biota is crucial for environmental risk assessment.
Purpose of the Study:
- To develop a miniaturized and efficient method for DEHP analysis in biota.
- To optimize sample preparation using matrix solid phase dispersion (MSPD).
- To determine DEHP levels in mussels and coastal waters.
Main Methods:
- Matrix solid phase dispersion (MSPD) using C18 as dispersant.
- Capillary liquid chromatography coupled to in-tube solid phase microextraction.
- Diode array detection for DEHP quantification.
- Optimization of eluting solvent (acetonitrile-water) and sample amount.
Main Results:
- Optimal recovery (91±15%) achieved with C18-Florisil® using 0.1 g sample and 2.6 mL eluent.
- Sample processing time under 30 minutes with no evaporation step required.
- Detection limit of 170 μg/kg for DEHP in mussels.
- Analysis of DEHP in mussels and associated coastal waters.
Conclusions:
- The developed MSPD method is rapid, efficient, and suitable for DEHP analysis in biota.
- The method facilitates environmental monitoring of phthalate contamination.
- Bioconcentration factors (BCFs) of DEHP in aquatic organisms warrant further investigation.
