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Related Concept Videos

IR Spectroscopy: Molecular Vibration Overview01:24

IR Spectroscopy: Molecular Vibration Overview

When Infrared (IR) radiation passes through a covalently bonded molecule, the bonds transition from lower to higher vibrational levels. The fundamental vibrational motions that result in infrared absorption can be classified as stretching or bending vibrations.
Stretching vibrations are vibrational motions that occur along the bond line, changing the bond length or distance between two bonded atoms. They are further distinguished as symmetric or asymmetric. In symmetric stretching, the...
IR Spectrum Peak Splitting: Symmetric vs Asymmetric Vibrations01:08

IR Spectrum Peak Splitting: Symmetric vs Asymmetric Vibrations

Identical bonds within a polyatomic group can stretch symmetrically (in-phase) or asymmetrically (out-of-phase). Similar to hydrogen bonding, these vibrations also influence the shape of the IR peak. Generally, asymmetric stretching frequencies are higher than symmetric stretching frequencies. For example, primary amines exhibit two distinct IR peaks between 3300–3500 cm−1 corresponding to the symmetric and asymmetric N-H stretching, while secondary amines exhibit a single stretching vibration...
Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
Atomic Force Microscopy01:08

Atomic Force Microscopy

Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
The AFM Probe
The probe is regarded as the heart of any AFM setup and comprises the...
X-ray Imaging01:24

X-ray Imaging

German physicist Wilhelm Röntgen (1845–1923) was experimenting with electrical current when he discovered that a mysterious and invisible "ray" would pass through his flesh but leave an outline of his bones on a screen coated with a metal compound. In 1895, Röntgen made the first durable record of the internal parts of a living human: an "X-ray" image (as it came to be called) of his wife’s hand. Scientists worldwide quickly began their own experiments with X-rays, and by 1900, X-ray was widely...
IR Frequency Region: X–H Stretching01:24

IR Frequency Region: X–H Stretching

In IR spectroscopy, signals produced by the X−H bonds (such as C−H, O−H, or N−H) can be observed in the frequency range of  2700–4000 cm–1. The C−H stretching vibration forms sharp bands in the region 2850–3000 cm–1. The presence of the O−H stretching vibration leads to the forming of an absorption band in the frequency range 3650–3200 cm−1. At the same time, N−H stretching can be confirmed by absorption bands in the 3500–3100 cm−1 range. Even though both O−H and N−H bonds vibrate at a similar...

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Related Experiment Video

Updated: May 18, 2026

Multimodal Nonlinear Hyperspectral Chemical Imaging Using Line-Scanning Vibrational Sum-Frequency Generation Microscopy
08:49

Multimodal Nonlinear Hyperspectral Chemical Imaging Using Line-Scanning Vibrational Sum-Frequency Generation Microscopy

Published on: December 1, 2023

Wide-field vibrational phase imaging.

Pascal Berto1, David Gachet, Pierre Bon

  • 1Institut Fresnel, CNRS UMR 7249, Aix-Marseille Université, Ecole Centrale Marseille, Campus de Saint Jérôme, F-13397 Marseille, France.

Physical Review Letters
|September 26, 2012
PubMed
Summary
This summary is machine-generated.

We developed a novel microscopy technique to capture the full complex field of molecular vibrations. This method enables background-free Raman spectroscopy for detailed molecular analysis.

More Related Videos

A Multimodal Wide-Field Fourier-Transform Raman Microscope
06:48

A Multimodal Wide-Field Fourier-Transform Raman Microscope

Published on: December 30, 2025

Related Experiment Videos

Last Updated: May 18, 2026

Multimodal Nonlinear Hyperspectral Chemical Imaging Using Line-Scanning Vibrational Sum-Frequency Generation Microscopy
08:49

Multimodal Nonlinear Hyperspectral Chemical Imaging Using Line-Scanning Vibrational Sum-Frequency Generation Microscopy

Published on: December 1, 2023

A Multimodal Wide-Field Fourier-Transform Raman Microscope
06:48

A Multimodal Wide-Field Fourier-Transform Raman Microscope

Published on: December 30, 2025

Area of Science:

  • Optical Microscopy
  • Spectroscopy
  • Molecular Imaging

Background:

  • Coherent and resonant molecular scatterers emit fields with both real and imaginary components.
  • Existing methods often require a reference beam or struggle with background noise.
  • Accurate retrieval of the complex field is crucial for detailed molecular characterization.

Purpose of the Study:

  • To introduce a wide-field microscopy method for retrieving the complex optical field of molecular scatterers.
  • To demonstrate a technique that does not necessitate a reference beam.
  • To enable background-free spectral analysis of molecular vibrations.

Main Methods:

  • Implementation of a wide-field microscopy technique.
  • Utilizing wave-front sensing for field retrieval.
  • Applying the method to wide-field coherent anti-Stokes Raman scattering (CARS) imaging.

Main Results:

  • Successful retrieval of the real and imaginary parts of the emitted field.
  • Demonstration of background-free coherent anti-Stokes Raman scattering imaging.
  • Acquisition of the complex anti-Stokes field by spectrally scanning molecular resonances.

Conclusions:

  • The proposed wave-front sensing microscopy method effectively retrieves the complex field of molecular scatterers.
  • This technique provides background-free access to molecular vibrational resonances.
  • It offers a powerful tool for detailed analysis of molecular bonds through Raman spectroscopy.