Related Experiment Video
Updated: May 18, 2026
HPLC Coupled with Chemical Fingerprinting for Multi-Pattern Recognition for Identifying the Authenticity of Clematidis Armandii Caulis
Published on: November 11, 2022
[Study on the HPCE fingerprint of Rehmannia glutinosa]
1Nanjing University of Chinese Medicine, Nanjing 210046, China.
Objective:
To establish the analytical method for the fingerprint of Rehmannia glutinosa by HPCE and compare the fingerprints of Rehmannia glutinosa and its processed products.
Methods:
Based on the mode of high performance capillary electrophoresis, 60 mmol/L sodium borate was used as buffer solution (5% MeOH, pH 9.5). The separation voltage was 20 kV and the detection wavelength was set at 210 nm. Catalpol was used as a reference standard, the chromatographic fingerprint were determined. The data were analyzed by fuzzy cluster and fingerprint similarity evaluation software was used to compare the similarity of samples.
Results:
HPCE fingerprints with 7 common peaks of Rehmannia glutinosa were established preliminarily. It was discovered that a small number of samples differed from others. Regarding to the fingerprints of Rehmannia glutinosa and its processed products, there were obvious differences in the relative areas of common peaks.
Conclusion:
The method is reliable, accurate and can be used for quality control of Rehmannia glutinosa.
