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Published on: November 21, 2019
Measurement of orientation and susceptibility ratios using a polarization-resolved second-harmonic generation
David G Winters1, David R Smith, Philip Schlup
1Department of Electrical and Computer Engineering, Colorado State University, Fort Collins, Colorado, USA.
Abstract:
Three-dimensional second-harmonic fields, sample orientation, and susceptibility ratios of biological samples are measured using polarization-resolved second-harmonic generation (SHG) microscopy. The three-dimensional (3D) polarization is gathered by measurement of a series of holograms for which excitation and analyzer polarizations are systematically varied, and the 3D SHG field is recovered through numerical back propagation. Harmonophore orientation is resolved in 3D from a sub-set of polarization-resolved SHG holograms. We further expand on previous approaches for the determination of susceptibility ratios, adding the calculation of multiple ratio values to allow intrinsic verification.
